Breaking biological barriers with a toothbrush
Breaking biological barriers with a toothbrush
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DOI：
10.1177/154405910708600816
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发表时间：
2007-08-01
影响因子：
7.6
通讯作者：
McNeil, P. L.
中科院分区：
文献类型：
--
作者：
Amano, K.;Miyake, K.;McNeil, P. L.
刷牙使口腔的上皮细胞和其他组织暴露于机械应力。在这里，我们研究了刷牙是否会诱导口腔中的上皮细胞和其他细胞类型的细胞创伤（质膜破坏）。刷牙的牙龈和舌头的大鼠导致了显着增加的细胞数量为阳性的破坏损伤的标志物。这些细胞包括牙龈上皮的所有层和舌头的骨骼肌中的细胞。此外，我们发现刷牙导致连接上皮细胞和骨骼肌细胞c-fos表达增加。然而，尽管观察到对细胞的个体损伤，但刷牙并未明显影响上皮屏障功能。我们的结论是刷牙破坏了口腔中的细胞质膜屏障，并激活了基因表达事件，这可能导致有利于牙龈健康的组织结构的局部适应性变化。
Toothbrushing exposes epithelia and other tissues of the oral cavity to mechanical stress. Here, we investigated whether brushing induces cell wounding-plasma membrane disruption-in epithelial and other cell types in the oral cavity. Brushing of the gingivae and tongues of rats resulted in a striking increase in the number of cells positive for a marker of disruption injury. These cells included those in all strata of the gingival epithelium, and in the skeletal muscle of the tongue. Additionally, we found that brushing resulted in an increase in c-fos expression by junctional epithelial and skeletal muscle cells. Epithelial barrier function, however, was not overtly affected by brushing, despite the observed individual injuries to cells. We concluded that brushing disrupts cell plasma membrane barriers in the oral cavity and activates gene expression events that may lead to local adaptive changes in tissue architecture beneficial to gingival health.