Cep57, a multidomain protein with unique microtubule and centrosomal localization domains
Cep57, a multidomain protein with unique microtubule and centrosomal localization domains
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DOI：
10.1042/bj20071501
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发表时间：
2008-06-01
影响因子：
4.1
通讯作者：
Somlyo, Avril V.
中科院分区：
文献类型：
--
作者：
Momotani, Ko;Khromov, Alexander S.;Somlyo, Avril V.
本研究证明了最近描述的中心体蛋白，Cep 57（中心体蛋白57）的不同功能域。内源性Cep 57蛋白和异位表达的全长蛋白质或N-末端卷曲螺旋结构域定位于中心体内部的γ-微管蛋白，这表明它是在两个中心粒或一个centromatrix组件。N-末端也可以与其他Cep 57分子的N-末端多聚化。C-末端含有第二个卷曲螺旋结构域，其直接结合MT（微管）。该结构域在体外使MT成核和成束。在体内也观察到这种活性，因为全长Cep 57或C末端的过表达在细胞中产生耐诺考达唑的MT电缆。基于目前的研究结果，我们建议，Cep 57作为一个链接，其N-末端锚定的中心粒或centromatrix和其C-末端MT。
The present study demonstrates different functional domains of a recently described centrosomal protein, Cep57 (centrosomal protein 57). Endogenous Cep57 protein and ectopic expression of full-length protein or the N-terminal coiled-coil domain localize to the centrosome internal to gamma-tubulin, suggesting that it is either on both centrioles or on a centromatrix component. The N-terminus can also multimerize with the N-terminus of other Cep57 molecules. The C-terminus contains a second coiled-coil domain that directly binds to MTs (microtubules). This domain both nucleates and bundles MTs in vitro. This activity was also seen in vivo, as overexpression of full-length Cep57 or the C-terminus generates nocodazole-resistant MT cables in cells. Based on the present findings, we propose that Cep57 serves as a link with its N-terminus anchored to the centriole or centromatrix and its C-terminus to MTs.