The expression of tomato prosystemin gene in tobacco plants highly affects host proteomic repertoire.
The expression of tomato prosystemin gene in tobacco plants highly affects host proteomic repertoire.
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DOI：
10.1016/j.jprot.2008.04.003
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发表时间：
2008-07
影响因子：
3.3
通讯作者：
M. Rocco;G. Corrado;Simona Arena;Chiara D’Ambrosio;C. Tortiglione;Stefano Sellaroli;M. Marra;R. Rao;A. Scaloni
中科院分区：
文献类型：
--
作者：
M. Rocco;G. Corrado;Simona Arena;Chiara D’Ambrosio;C. Tortiglione;Stefano Sellaroli;M. Marra;R. Rao;A. Scaloni
系统素是从番茄中分离的一种十八肽，是参与害虫攻击的局部和系统反应的主要信号分子，由一组防御基因的激活引起。它来源于前系统素的加工，这是一种近200个氨基酸的激素原。在其他茄科物种中发现了前系统素直向同源物，但在烟草中未发现，烟草中存在与番茄系统素功能上但结构上无关的富含羟脯氨酸的肽。导致从蛋白质前体释放信号肽的分子事件在植物中是未知的;信号分子家族的发生表明伤口反应的启动可能涉及不同的处理机制。先前已经表明，来自工程番茄前系统素基因的蛋白质产物在烟草中加工，从而表明负责其翻译后修饰的组分存在于该物种中。通过对转化烟草叶片的蛋白质组进行2-DE分析，证明番茄前系统素基因的组成型表达对宿主蛋白质合成有很大影响。特别是，工程植物显示出许多差异合成的蛋白质，这些蛋白质通过PMF MALDI-TOF和µLC-ESI-IT-MS/MS实验鉴定为参与保护免受病原体和氧化胁迫或碳/能量代谢的多肽种类。观察到的显着差异，在过度生产的蛋白质与以前的数据报告的系统蛋白工程番茄植物。我们的研究结果强烈支持需要使用蛋白质组学方法在植物组织的系统分析，以调查在转基因植物表达的转基因编码的信号分子的实质等同的原则。
Systemin, an octadecapeptide isolated from tomato, is a primary signal molecule involved in the local and systemic responses to pest attack, elicited by activation of a set of defence genes. It derives from processing of prosystemin, a prohormone of almost 200 amino acids. Prosystemin orthologues have been found in other Solanaceae species but not in tobacco, where are present hydroxyproline-rich peptides functionally but not structurally related to tomato systemin. Molecular events leading to the release of signalling peptides from protein precursors are unknown in plants; the occurrence of a family of signal molecules suggests that initiation of wound response may involve different processing mechanisms. It has been previously shown that the protein product from an engineered tomato prosystemin gene is processed in tobacco, thus suggesting that the components responsible for its post-translational modifications are present in this species. By analyzing analysing the proteome repertoire of transformed tobacco plant leaves with 2-DE, here we demonstrate that the constitutive expression of the tomato prosystemin gene highly affected host protein synthesis. In particular, engineered plants showed a number of differentially synthesized proteins that were identified by PMF MALDI-TOF and µLC-ESI-IT-MS/MS experiments as polypeptide species involved in protection from pathogens and oxidative stress, or in carbon/energy metabolism. Significant differences in over-produced proteins were observed with respect to previous data reported on systemin-engineered tomato plants. Our results strongly support the need of using proteomic approaches during systematic analysis of plant tissues to investigate the principle of substantial equivalence in transgenic plants expressing a transgene coding for a signalling molecule.