An electrochemiluminescence aptasensor for analysis of bisphenol A based on carbon nanodots composite as co-reaction of Ru(bpy)32+ nanosheets
An electrochemiluminescence aptasensor for analysis of bisphenol A based on carbon nanodots composite as co-reaction of Ru(bpy)32+ nanosheets
复制标题
基于碳纳米点复合物与 Ru(bpy)(3)(2 ) 纳米片共反应的电化学发光适体传感器用于分析双酚 A
DOI：
10.1016/j.electacta.2019.07.035
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发表时间：
2019-10-01
影响因子：
6.6
通讯作者：
You, Tianyan
中科院分区：
文献类型：
--
作者：
Liu, Xiaohong;Luo, Lijun;You, Tianyan
以聚乙亚胺功能化氮掺杂碳纳米点（NCDs@PEI）复合材料为共聚物，Ru(bpy)(3)（2+）纳米片（RuNSs）为发光材料，构建了电化学发光（ECL）适体传感器，用于双酚A （BPA）的特异性分析。由于NCDs和PEI对RuNSs的双重改善作用，所开发的适配传感器具有良好的ECL性能。为了进一步提高RuNSs-NCDs@PEI系统对BPA检测的选择性，我们创新性地引入BPA适体，构建了一种新型ECL适体传感器。在结构类似物的干扰下，与不含适体和随机DNA的适体传感器相比，构建的适体传感器对BPA的检测具有更高的特异性。所制备的ECL传感器具有1.0 × 10(-10) ~ 1.0 × 10(-4) mol L-1的宽线性范围和3.3 × 10(-11) mol L-1的低检出限，具有良好的稳定性和重复性。最后，将ECL传感器用于水样中BPA的分析，用高效液相色谱-紫外光谱（HPLC-UV）方法对所得结果进行了验证，验证了其良好的准确性。该传感器为水样中BPA的检测提供了新的平台。(C) 2019年Elsevier Ltd.出版
An electrochemiluminescence (ECL) aptasensor based on poly(ethylenimine) functionalized nitrogen-doped carbon nanodots (NCDs@PEI) composite as co-reactant, and Ru(bpy)(3)(2+) nanosheets (RuNSs) as luminophor was constructed for the specific analysis of bisphenol A (BPA). On account of the double improvement functions of NCDs and PEI on RuNSs, the as-developed aptasensor exhibited superior ECL properties. In order to further enhance the selectivity of the RuNSs-NCDs@PEI system for BPA detection, we innovatively introduced the BPA aptamer to construct a novel ECL aptasensor. Compared with that without aptamer and with random DNA, the constructed aptasensor performed higher specificity for BPA determination under the interference of structural analogues. The as-prepared ECL aptasensor exhibited a wide linear range of 1.0 x 10(-10) - 1.0 x 10(-4) mol L-1, and a relatively low detection limit of 3.3 x 10(-11) mol L-1 for BPA detection with good stability and repeatability. Finally, when the ECL aptasensor made use of BPA analysis in water samples, the obtained results were confirmed by high performance liquid chromatography-ultraviolet spectrum (HPLC-UV) method, verifying its good accuracy. The novel aptasensor provided a new sensing platform for BPA in water samples. (C) 2019 Published by Elsevier Ltd.