Expression of glucose transporters and hexokinase II in cholangiocellular carcinoma compared using [18F]-2-fluro-2-deoxy-D-glucose positron emission tomography
Expression of glucose transporters and hexokinase II in cholangiocellular carcinoma compared using [18F]-2-fluro-2-deoxy-D-glucose positron emission tomography
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DOI：
10.1111/j.1349-7006.2007.00683.x
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发表时间：
2008-02-01
期刊：
影响因子：
5.7
通讯作者：
Endo, Keigo
中科院分区：
文献类型：
--
作者：
Paudyal, Bishnuhari;Oriuchi, Noboru;Endo, Keigo
胆管细胞癌（CCC）已被报道具有高葡萄糖摄取，然而，葡萄糖进入这些细胞的机制仍然不清楚。我们研究了[F-18]-2-氟-2-脱氧-D-葡萄糖（F-18-FDG）摄取与葡萄糖易化转运蛋白（Glut）和己糖激酶（HK）II表达之间的关系，以及不同组织学类型CCC表达之间的相关性。应用免疫组化方法对26例CCC患者术前或活检前行全身F-18-FDG正电子发射断层扫描（PET）检查，检测Glut（1-5）和HK Ⅱ的表达。CCC中Glut 1、Glut 2、Glut 3和HK II的阳性率分别为81%、54%、19%和77%。Glut 1、Glut 2、Glut 3和HK II在中分化和低分化CCC中的检出率高于高分化CCC。观察到F-18-FDG摄取与Glut 1和HK II染色评分之间存在显著相关性（P = 0.02，rho = 0.45和P = 0.001，rho = 0.59）。Glut 1和HK II的染色评分也显著相关（P = 0.002，rho = 0.3）。多因素回归分析显示淋巴结转移与F-18-FDG摄取独立相关。我们的研究表明Glut 1和HK II的表达与F-18-FDG摄取之间存在显著相关性，表明Glut 1是CCC中表达的主要葡萄糖转运蛋白，HK II有助于葡萄糖代谢的增加，尤其是在中度和低分化CCC中。
Cholangiocellular carcinoma (CCC) has been reported to have a high glucose uptake; however, the mechanism of glucose entry into these cells is still unclear. We investigated the relationship between [F-18]-2-fluro-2-deoxy-D-glucose (F-18-FDG) uptake and the expression of facilitative glucose transporters (Glut) and hexokinase (HK) II, as well as the association between the expression of different histological types of CCC. The expression of Glut (1-5) and HK II was studied using immunohistochemistry of 26 patients with CCC who underwent whole-body F-18-FDG positron emission tomography before surgery or biopsy. CCC expressed immunohistochemically detectable Glut 1 in 81%, Glut 2 in 54%, Glut 3 in 19%, and HK II in 77% of the total cases. Glut 1, Glut 2, Glut 3, and HK II were more often detected in moderately differentiated and poorly differentiated than in well-differentiated CCC. A significant correlation was observed between F-18-FDG uptake and the staining scores of Glut 1 and HK II (P = 0.02, rho = 0.45 and P = 0.001, rho = 0.59). The staining scores of Glut 1 and HK II were also significantly correlated (P = 0.002, rho = 0.3). Multivariate regression analysis revealed that lymph-node metastasis was independently associated with F-18-FDG uptake. Our study showed a significant association between the expression of Glut 1 and HK II with F-18-FDG uptake, indicating that Glut 1 is a major glucose transporter expressed in CCC and that HK II contributes to the increased metabolism of glucose, especially in moderately and poorly differentiated CCC.