INHALATION OF 2% HYDROGEN IMPROVES SURVIVAL RATE AND ATTENUATES SHEDDING OF VASCULAR ENDOTHELIAL GLYCOCALYX IN RATS WITH HEAT STROKE
INHALATION OF 2% HYDROGEN IMPROVES SURVIVAL RATE AND ATTENUATES SHEDDING OF VASCULAR ENDOTHELIAL GLYCOCALYX IN RATS WITH HEAT STROKE
复制标题
吸入2%氢可提高热休克大鼠存活率和减少血管内皮糖萼脱落
DOI：
10.1097/shk.0000000000001797
复制
发表时间：
2021-10-01
期刊：
影响因子：
3.1
通讯作者：
Nakajima, Yoshiki
中科院分区：
文献类型：
--
作者：
Truong, Sang Kien;Katoh, Takasumi;Nakajima, Yoshiki
中暑的特点是过度的氧化应激和炎症反应，这两者都与血管内皮糖萼脱落和中暑死亡率有关。虽然氢分子具有抗氧化和抗炎作用，但其对中暑血管内皮糖萼的影响尚未被研究。因此，本研究旨在探讨氢吸入对中暑大鼠血管内皮糖萼厚度和存活的影响。共有98只Wistar大鼠被分配到实验中。使用温度为40摄氏度、湿度为60%的热控室诱导中暑。制备后，经加热过程麻醉的大鼠吸入0%、2%或4%氢气稳定1小时，并维持至实验结束。除了存活率评估外，还收集血液样本和左心室来评估血管内皮糖萼的厚度和相关生物标志物。结果表明，2%的氢气能显著提高热休克大鼠的存活率，并能部分保存内皮糖萼的厚度。此外，血清内毒素、syndecan-1、丙二醛和肿瘤坏死因子α水平降低，而超氧化物歧化酶水平升高，表明吸入2%氢通过其抗氧化和抗炎作用减轻了血管内皮糖萼的损伤。
Heat stroke is characterized by excessive oxidative stress and inflammatory responses, both of which are implicated in vascular endothelial glycocalyx shedding and heat-stroke mortality. Although molecular hydrogen has antioxidation and anti-inflammatory potency, its effect on the vascular endothelial glycocalyx in heat stroke has not been examined. Therefore, the aim of this study was to investigate the influence of hydrogen inhalation on the survival and thickness of the vascular endothelial glycocalyx of rats subjected to heat stroke. Altogether, 98 Wistar rats were assigned to the experiments. A heat-controlled chamber set at 40 degrees C temperature and 60% humidity was used to induce heat stroke. After preparation, the anesthetized rats that underwent the heating process were subjected to an hour of stabilization in which 0%, 2%, or 4% hydrogen gas was inhaled and maintained until the experiment ended. In addition to survival rate assessments, blood samples and left ventricles were collected to evaluate the thickness of the vascular endothelial glycocalyx and relevant biomarkers. The results showed that 2% hydrogen gas significantly improved survival in the heat-stroked rats and partially preserved the thickness of the endothelial glycocalyx. In addition, serum levels of endotoxin, syndecan-1, malondialdehyde, and tumor necrosis factor-alpha decreased, whereas superoxide dismutase levels increased, indicating that inhalation of 2% hydrogen attenuated the damage to the vascular endothelial glycocalyx through its antioxidative and anti-inflammatory effects.