Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing
Global Dynamic Molecular Profiling of Stomatal Lineage Cell Development by Single-Cell RNA Sequencing
复制标题
通过单细胞 RNA 测序对气孔谱系细胞发育进行全局动态分子分析
DOI：
10.1016/j.molp.2020.06.010
复制
发表时间：
2020-08-03
期刊：
影响因子：
27.5
通讯作者：
Sun, Xuwu
中科院分区：
文献类型：
--
作者：
Liu, Zhixin;Zhou, Yaping;Sun, Xuwu
气孔谱系细胞发育的调控已被广泛研究。然而，基于单细胞转录组分析的这种生物过程的全面表征尚未报道。在这项研究中，我们进行了RNA测序的12 844个单独的细胞从子叶的5天大的拟南芥幼苗。我们确定了11个细胞簇，主要对应于细胞在特定的气孔发育阶段，使用一系列的标记基因。对这些细胞簇中具有最高可变表达的基因的比较分析揭示了调节从分生组织样母细胞到保卫母细胞的发育的转录网络。通过伪时间分析的标记基因的发育动力学检查揭示了这些基因之间的潜在相互作用。总的来说，我们的研究打开了大门，了解如何确定新的标记基因参与调节气孔谱系细胞的发展。
The regulation of stomatal lineage cell development has been extensively investigated. However, a comprehensive characterization of this biological process based on single-cell transcriptome analysis has not yet been reported. In this study, we performed RNA sequencing on 12 844 individual cells from the cotyledons of 5-day-old Arabidopsis seedlings. We identified 11 cell clusters corresponding mostly to cells at specific stomatal developmental stages using a series of marker genes. Comparative analysis of genes with the highest variable expression among these cell clusters revealed transcriptional networks that regulate development from meristemoid mother cells to guard mother cells. Examination of the developmental dynamics of marker genes via pseudo-time analysis revealed potential interactions between these genes. Collectively, our study opens the door for understanding how the identified novel marker genes participate in the regulation of stomatal lineage cell development.