MOLECULAR-BASIS OF PLANT VIRAL VIRULENCE - THE COMPLETE NUCLEOTIDE-SEQUENCE OF AN ATTENUATED STRAIN OF TOBACCO MOSAIC-VIRUS
MOLECULAR-BASIS OF PLANT VIRAL VIRULENCE - THE COMPLETE NUCLEOTIDE-SEQUENCE OF AN ATTENUATED STRAIN OF TOBACCO MOSAIC-VIRUS
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DOI：
10.1093/nar/13.15.5585
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
OKADA, Y
中科院分区：
文献类型：
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作者：
NISHIGUCHI, M;KIKUCHI, S;OKADA, Y
测定了烟草花叶病毒（TMV）弱毒株L11A的全基因组序列。该菌株能够在番茄植株中繁殖而不引起任何显著症状，但保护它们免受毒性TMV菌株的感染。与已发表的TMV L（L11A强毒祖先株系）全基因组序列比较，发现L11A基因组中有10个碱基发生了替换。其中7个发生在同相密码子的第三个字母中，并且不影响氨基酸。只有三个，这是在共同的阅读框架的130 K和180 K蛋白质，导致氨基酸的变化。与从L到L11A连续分离的中间株L11的RNA部分序列的结果一起，观察到在核苷酸位置1117处的一个碱基从L变为L111，并且在位置2349和2754处的两个碱基从L11A变为L11A。
The total genome sequence of L11A, an attenuated strain of tobacco mosaic virus (TMV), has been determinea. This strain is able to multiply in tomato plants without inducing any remarkable symptoms, but to protect them from later infection with virulent TMV strains. When compared with the recently published total genome sequence of TMV L (the virulent ancestrastrain of L11A) ten base substitutions were found in the L11A genome. Seven of these occurred in the third letters of in-phase codons and did not influence amino acids. Only three, which were in the common reading frame for both the 130K and 180K proteins, resulted in amino acid changes. Together with the result of the partial sequence of RNA of L11, an intermediate strain in sequential isolation from L to L11A, it is observed that one base at the nucleotide position 1117 is changed from L to L11and two bases at the positions 2349 and 2754 are changed from L11to L A.