Functionalized Single-Walled Carbon Nanotubes and Nanographene Oxide to Overcome Antibiotic Resistance in Tetracycline-Resistant Escherichia coli
Functionalized Single-Walled Carbon Nanotubes and Nanographene Oxide to Overcome Antibiotic Resistance in Tetracycline-Resistant Escherichia coli
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DOI：
10.1021/acsanm.0c00677
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发表时间：
2020-04-24
影响因子：
5.9
通讯作者：
Ellison, Mark D.
中科院分区：
文献类型：
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作者：
Carver, Jordan A.;Simpson, Audrey L.;Ellison, Mark D.
抗生素耐药性是一个日益严重的重大公共卫生问题。这项工作研究了使用两种不同的碳纳米材料，单壁碳纳米管（SWNTs）和纳米石墨烯氧化物（NGO），作为一种将抗生素四环素输送到具有外排泵抗性机制的大肠杆菌菌株的手段。研究发现，携带四环素的swnt和NGO均能抑制耐药菌株，尽管四环素的递送量远低于游离四环素的最低抑制浓度。发现四环素附着在纳米材料上是抑制细菌生长所必需的，这表明纳米材料正在将抗生素运送到细胞中并破坏外排泵。研究人员观察到，与氧化石墨烯相比，单壁碳纳米管在递送四环素方面具有更大的功效，这归因于其针状形状。这项工作证明了碳纳米材料作为抗生素递送载体的使用以及纳米材料形状对其功效的影响。更重要的是，这表明纳米材料可以成功延长现有抗生素的寿命，使其成为对抗外排泵机制介导的抗生素耐药性的重要工具。
Antibiotic resistance is a significant and growing public health problem. This work investigated the use of two different carbon nanomaterials, single-walled carbon nanotubes (SWNTs) and nanographene oxide (NGO), as a means of delivering the antibiotic tetracycline to a strain of Escherichia coli bacterium with an efflux pump resistance mechanism. Both SWNTs and NGO carrying tetracycline were found to inhibit the resistant strain of Escherichia coli, though the amount of tetracycline delivered was much lower than the minimum inhibitory concentration of free tetracycline. Attachment of the tetracycline to the nanomaterials was found to be necessary for the inhibition of bacterial growth, indicating that the nanomaterials were transporting the antibiotic into the cells and subverting the efflux pump. SWNTs were observed to have greater efficacy in delivering tetracycline than graphene oxide, which is attributed to the SWNTs' needle-like shape. This work demonstrates both the use of carbon nanomaterials as antibiotic-delivery vehicles and the effect of nanomaterial shape on their efficacy. More importantly, it demonstrates that nanomaterials can successfully extend the life of existing antibiotics, making them an important tool for combatting antibiotic resistance mediated by an efflux pump mechanism.