Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing.
Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing.
复制标题
用于研究细菌疾病和抗菌药物测试的梅洛尼菌感染模型。
DOI：
10.1080/21505594.2015.1135289
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发表时间：
2016-04-02
期刊：
影响因子：
5.2
通讯作者：
Proft T
中科院分区：
文献类型：
--
作者：
Tsai CJ;Loh JM;Proft T
梅氏小蛾(大蜡蛾或蜂窝蛾)已被引入作为研究微生物感染的替代模型。梅氏杆菌幼虫可以很容易、廉价地大量获得，而且使用简单，因为它们不需要特殊的实验室设备。没有伦理限制，而且它们的生命周期短，是进行大规模研究的理想选择。虽然昆虫缺乏适应性免疫反应，但它们的先天免疫反应与脊椎动物的免疫反应有显著的相似之处。本文综述了有关梅氏革兰氏菌免疫系统的最新研究进展，并对梅隆革兰氏菌如何被用于研究革兰氏阳性和革兰氏阴性细菌的毒力进行了广泛的综述。此外，还讨论了用梅氏革兰氏菌评价抗菌药物的疗效和实验性噬菌体治疗。该综述最后对目前梅氏革兰氏菌感染模型的局限性进行了批判性评估。
Galleria mellonella (greater wax moth or honeycomb moth) has been introduced as an alternative model to study microbial infections. G. mellonella larvae can be easily and inexpensively obtained in large numbers and are simple to use as they don't require special lab equipment. There are no ethical constraints and their short life cycle makes them ideal for large-scale studies. Although insects lack an adaptive immune response, their innate immune response shows remarkable similarities with the immune response in vertebrates. This review gives a current update of what is known about the immune system of G. mellonella and provides an extensive overview of how G. mellonella is used to study the virulence of Gram-positive and Gram-negative bacteria. In addition, the use of G. mellonella to evaluate the efficacy of antimicrobial agents and experimental phage therapy are also discussed. The review concludes with a critical assessment of the current limitatons of G. mellonella infection models.