The entire nucleotide sequence of a TT virus isolate from the united states (TUSO1): Comparison with reported isolates and phylogenetic analysis
The entire nucleotide sequence of a TT virus isolate from the united states (TUSO1): Comparison with reported isolates and phylogenetic analysis
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DOI：
10.1006/viro.1999.9769
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发表时间：
1999-07-05
期刊：
影响因子：
3.7
通讯作者：
Mayumi, M
中科院分区：
文献类型：
--
作者：
Okamoto, H;Nishizawa, T;Mayumi, M
日本报道了一种无包膜单链 DNA 病毒，称为 TT 病毒 (TTV)，与病因不明的肝炎有关。最近，基因型 1 的原型 TTV 分离株 (TA278) 被证明具有包含 3852 个核苷酸的环状基因组。从美国的一名献血者身上回收了 TTV 分离株（TUS01），并测定了其 3818 个核苷酸的完整环状核苷酸序列。它拥有两个开放阅读框，分别编码761和156个氨基酸。 TUS01 与来自日本的 TA278 分离株有 60.5% 的核苷酸序列相同，长了 35 nt。 1203nt的非编码区序列保守，相似度为83.4%。两个开放阅读框的序列保存率要低得多；其中一个的核苷酸和氨基酸序列相似度分别为 54.8% 和 37.0%，另一个的相似度分别为 55.5% 和 38.8%。通过比较来自不同国家的239个TTV分离株的222个核苷酸的部分序列，至少识别出11个序列差异>30%的基因型。 TUS01被推断为基因型11，此前未见报道。非编码区的保守序列可用作通过聚合酶链式反应灵敏检测TTV DNA的引物。编码区中的不同序列可用作区分各种 TTV 基因型的引物。 (C) 1999 年学术出版社。
A nonenveloped and single-stranded DNA virus designated TT virus (TTV) has been reported from Japan in association with hepatitis of unknown etiology. Very recently, the prototype TTV isolate (TA278) of genotype 1 is proven to have a circular genome with 3852 nucleotides. A TTV isolate (TUS01) was recovered from a blood donor in the United States, and its entire circular nucleotide sequence of 3818 nucleotides was determined. It possessed two open reading frames coding for 761 and 156 amino acids, respectively. TUS01 shared 60.5% of the nucleotide sequence with the TA278 isolate from Japan that was longer by 35 nt. The sequence of the noncoding region of 1203 nt was conserved with a similarity of 83.4%. Sequence preservation was much lower for the two open reading frames; nucleotide and amino acid sequences were 54.8 and 37.0% similar, respectively, for one and 55.5 and 38.8% similar for the other. By comparison of a partial sequence of 222 nucleotides among 239 TTV isolates available from various countries, at least 11 genotypes with sequence divergence of >30% were recognized. TUS01 was deduced to be of genotype 11, which has not been reported before. Conserved sequences in the noncoding region could be used as primers for sensitively detecting TTV DNA by polymerase chain reaction. Divergent sequences in coding regions would be useful as primers for distinguishing various TTV genotypes. (C) 1999 Academic Press.