SerpinE2, a poor biomarker of endometrial cancer, promotes the proliferation and mobility of EC cells
SerpinE2, a poor biomarker of endometrial cancer, promotes the proliferation and mobility of EC cells
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DOI：
10.3233/cbm-160442
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发表时间：
2017-01-01
影响因子：
3.1
通讯作者：
Lu, Lin
中科院分区：
文献类型：
--
作者：
Shen, Yuan;Wang, Xiaoyu;Lu, Lin
Serpine2途径在进化上是保守的，在肿瘤的发生中起着重要作用。Serpine2(一种与泛素相关的小修饰物)和泛素一样，将Serpine2蛋白连接到目标蛋白的赖氨酸残基上。在几种肿瘤中发现了Serpine2的过度表达。在这里，我们用免疫组织化学方法检测了72例EC组织中Serpine2的水平，以评估Serpine2在EC预后中的作用。同时，我们用siRNA在HTB111和Ishikawa EC细胞系中敲除Serpine2，并用四甲基偶氮唑盐比色法、Annexin V/PI细胞凋亡率实验、伤口划痕实验和Transwell实验分析细胞存活率和迁移能力的变化。Kaplan-Meier分析显示Serpine2水平与EC预后呈负相关。沉默Serpine2诱导细胞凋亡，降低迁移能力。我们的数据表明Serpine2在EC中作为癌基因发挥作用。
The SerpinE2 pathway is evolutionarily conserved and plays an important role in tumorigenesis. SerpinE2 ( a small ubiquitin-related modifier), like ubiquitin, conjugates SerpinE2 proteins onto lysine residues of target proteins. SerpinE2 overexpression has been found in several tumors. Here, we detected the level of SerpinE2 in 72 samples of EC tissue using immunohistochemistry to assess the role of SerpinE2 in EC prognosis. Meanwhile, we knocked down SerpinE2 by siRNA in the HTB111 and Ishikawa EC cell lines and analyzed the viability and mobility change using an MTT assay, an annexin V/PI apoptosis assay, a wound scratch test and a transwell assay. A Kaplan-Meier analysis indicated a negative correlation between the level of SerpinE2 and the EC prognosis. Silencing SerpinE2 induced cell apoptosis and reduced the migration ability. Our data suggest SerpinE2 works as an oncogene in EC.