Production of Acetone-Butanol-Ethanol (ABE) in Direct Fermentation of Cassava by Clostridium saccharoperbutylacetonicum N1-4
Production of Acetone-Butanol-Ethanol (ABE) in Direct Fermentation of Cassava by Clostridium saccharoperbutylacetonicum N1-4
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DOI：
10.1007/s12010-009-8770-1
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发表时间：
2010-05-01
影响因子：
3
通讯作者：
Kobayashi, Genta
中科院分区：
文献类型：
--
作者：
Thang, Vu Hong;Kanda, Kohzo;Kobayashi, Genta
研究了木薯淀粉和木薯片利用糖化丁基丙酮梭菌N1-4发酵时的丙酮-丁醇-乙醇(ABE)发酵特性。利用1-L发酵罐进行分批发酵的结果表明，糖化丁基丙酮C菌N1-4是一株高淀粉分解菌株，能够高效地利用木薯淀粉和木薯切片生产溶剂，与以葡萄糖为原料时相当。分批发酵木薯淀粉和木薯片的总溶剂分别为21.0g/L和19.4g/L，而用葡萄糖分批发酵的总溶剂为24.2g/L。在相同条件下，木薯淀粉发酵的溶剂产率比玉米和西米淀粉发酵的溶剂产率高42%~63%。在木薯淀粉和木薯片的发酵中，丁醇的最高浓度分别为16.9g/L和15.5g/L。木薯淀粉发酵的溶剂产率和丁醇产率(以潜在葡萄糖计)分别为0.33和0.41，木薯片发酵的溶剂产率和丁醇产率分别为0.30和0.38。
In this work, acetone-butanol-ethanol (ABE) fermentation characteristics of cassava starch and cassava chips when using Clostridium saccharoperbutylacetonicum N1-4 was presented. The obtained results in batch mode using a 1-L fermenter showed that C. saccharoperbutylacetonicum N1-4 was a hyperamylolytic strain and capable of producing solvents efficiently from cassava starch and cassava chips, which was comparable to when glucose was used. Batch fermentation of cassava starch and cassava chips resulted in 21.0 and 19.4 g/L of total solvent as compared with 24.2 g/L of total solvent when using glucose. Solvent productivity in fermentation of cassava starch was from 42% to 63% higher than that obtained in fermentation using corn and sago starches in the same condition. In fermentation of cassava starch and cassava chips, maximum butanol concentration was 16.9 and 15.5 g/L, respectively. Solvent yield and butanol yield (based on potential glucose) was 0.33 and 0.41, respectively, for fermentation of cassava starch and 0.30 and 0.38, respectively for fermentation using cassava chips.