Central origin of the antinociceptive action of botulinum toxin type A
Central origin of the antinociceptive action of botulinum toxin type A
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DOI：
10.1016/j.pbb.2009.08.012
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发表时间：
2009-12-01
影响因子：
3.6
通讯作者：
Lackovic, Zdravko
中科院分区：
文献类型：
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作者：
Bach-Rojecky, Lidija;Lackovic, Zdravko
在这里，我们提供了行为证据的轴突运输和A型肉毒杆菌毒素（M-A）的抗伤害性作用的中枢起源。在大鼠中，我们研究了BTX-A对单侧重复肌肉注射酸性盐水（pH 4.0）引起的“镜像痛”的有效性。由于实验证据表明，由酸性生理盐水引起的双侧疼痛是中枢性的，因此外周应用BTX-A对这种类型的疼痛应该没有影响。然而，在这里，我们证明了单侧皮下BTX-A（5 U/kg）应用减少了同侧和对侧的疼痛。当注射到远端切断的坐骨神经的近端部分时，BTX-A仍然减轻了对侧的疼痛。秋水仙碱，轴突运输阻滞剂，当注射到同侧坐骨神经，阻止了周围注射BTX-A对两侧的影响。此外，当BTX-A（1 U/kg）鞘内应用于腰椎脑脊液中时，双侧痛觉过敏也减少。结果表明，逆行轴突运输的必要性和中枢神经系统的参与BTX-A的抗伤害活性。(C)2009 Elsevier Inc. All rights reserved.
Here we provide behavioural evidence for an axonal transport and the central origin of the antinociceptive effect of botulinum toxin type A (M-A). In rats we investigated the effectiveness of BTX-A on "mirror pain" induced by unilateral repeated intramuscular acidic saline injections (pH 4.0). Since experimental evidence suggest that bilateral pain induced by acidic saline is of central origin, peripheral application of BTX-A should have no effect on this type of pain. However, here we demonstrated that the unilateral subcutaneous BTX-A (5 U/kg) application diminished pain on the ipsilateral, and on the contralateral side too. When injected into the proximal part of a distally cut sciatic nerve, BTX-A still reduced pain on the contralateral side. Colchicine, an axonal transport blocker, when injected into the ipsilateral sciatic nerve, prevented the effect of the peripheral BTX-A injection on both sides. Additionally, when BTX-A (1 U/kg) was applied intrathecally in the lumbar cerebrospinal fluid, the bilateral hyperalgesia was also reduced. The results demonstrate the necessity of retrograde axonal transport and involvement of the central nervous system for the antinociceptive activity of BTX-A. (C) 2009 Elsevier Inc. All rights reserved.