Genetic diversity of Anopheles coustani in high malaria transmission foci in southern and central Africa
Genetic diversity of Anopheles coustani in high malaria transmission foci in southern and central Africa
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南部和中部非洲疟疾高传播灶库斯坦按蚊的遗传多样性
DOI：
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
D. Norris
中科院分区：
文献类型：
--
作者：
Ilinca I. Ciubotariu;Christine M. Jones;Tamaki Kobayashi;T. Bobanga;Mbanga Muleba;J. C. Pringle;J. Stevenson;G. Carpi;D. Norris
尽管在整个撒哈拉以南非洲持续开展疟疾控制工作，但疟疾仍然是一个巨大的公共卫生问题。目前的干预措施，如室内滞留喷洒杀虫剂和使用驱虫蚊帐，都是针对主要是内噬性和嗜内性的主要疟疾病媒。虽然这些控制措施使疟疾病例大幅减少，并继续影响室内传播，但疟疾传播的替代媒介的重要性在很大程度上被忽视。库斯坦按蚊是一种未被充分研究的疟疾媒介，以前被认为主要表现出嗜动物行为。然而，最近来自非洲各地的研究揭示了这种与生态相似的按蚊物种对人类疟疾传播的贡献。像许多这些未被充分研究的物种一样，一个。库斯坦氏疟原虫具有比以前认识到的更大的嗜中性倾向，通常是内噬和外噬的，并携带恶性疟原虫子孢子。这些最近的事态发展突出表明，需要在该物种的整个地理范围内进行更多的研究，并有可能需要控制这种媒介。本研究的目的是探讨安氏按蚊的遗传变异。在赞比亚Nchelenge区和刚果民主共和国上加丹加省Kashobwe和Kilwa卫生区的高传播环境中，对库斯坦蚊和这种按蚊物种促进疟疾寄生虫传播的潜力进行了研究。形态学鉴定为An。通过PCR和测序分析在这些研究地点的户外捕获的库斯坦标本的物种鉴定和血粉来源，并通过ELISA和qPCR确定疟原虫感染。50份标本经鉴定为An。线粒体DNA细胞色素c氧化酶亚基I（COI）和核糖体内转录间隔区2（ITS2）的分析表明，此外，COI和ITS2序列的最大似然系统发育分析揭示了两个不同的系统发育组在这个相对较小的区域集合。我们的研究结果表明，无论是一个。库斯坦群体具有嗜亲性和外食性习性，并经常与恶性疟原虫接触，这表明这种潜在的替代疟疾媒介可能无法在赞比亚北方和刚果民主共和国南部进行目前的媒介控制。本研究为深入研究安氏按蚊的生物学特性和遗传多样性奠定了基础。coustani及其对这些地区疟疾传播的贡献。
Despite ongoing malaria control efforts implemented throughout sub-Saharan Africa, malaria remains an enormous public health concern. Current interventions such as indoor residual spraying with insecticides and use of insecticide-treated bed nets are aimed at targeting the key malaria vectors that are primarily endophagic and endophilic. While these control measures have resulted in a substantial decline in malaria cases and continue to impact indoor transmission, the importance of alternative vectors for malaria transmission has been largely neglected. Anopheles coustani, an understudied vector of malaria, is a species previously thought to exhibit mostly zoophilic behavior. However, recent studies from across Africa bring to light the contribution of this and ecologically similar anopheline species to human malaria transmission. Like many of these understudied species, An. coustani has greater anthropophilic tendencies than previously appreciated, is often both endophagic and exophagic, and carries Plasmodium falciparum sporozoites. These recent developments highlight the need for more studies throughout the geographic range of this species and the potential need to control this vector. The aim of this study was to explore the genetic variation of An. coustani mosquitoes and the potential of this Anopheles species to contribute to malaria parasite transmission in high transmission settings in Nchelenge District, Zambia, and the Kashobwe and Kilwa Health Zones in Haut-Katanga Province, the Democratic Republic of the Congo (DRC). Morphologically identified An. coustani specimens that were trapped outdoors in these study sites were analyzed by PCR and sequencing for species identification and blood meal sources, and malaria parasite infection was determined by ELISA and qPCR. Fifty specimens were confirmed to be An. coustani by the analysis of mitochondrial DNA cytochrome c oxidase subunit I (COI) and ribosomal internal transcribed spacer region 2 (ITS2). Further, maximum likelihood phylogenetic analysis of COI and ITS2 sequences revealed two distinct phylogenetic groups within this relatively small regional collection. Our findings indicate that both An. coustani groups have anthropophilic and exophagic habits and come into frequent contact with P. falciparum, suggesting that this potential alternative malaria vector might elude current vector controls in Northern Zambia and Southern DRC. This study sets the groundwork for more thorough investigations of bionomic characteristics and genetic diversity of An. coustani and its contribution to malaria transmission in these regions.
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DOI：
10.1385/1-59259-271-6:01
发表时间：
2002
期刊：
Methods in molecular medicine
影响因子：
--
作者：
Beier,JohnC
通讯作者：
Beier,JohnC
DOI：
10.4269/ajtmh.2005.73.336
发表时间：
2005-08-01
影响因子：
3.3
作者：
Kent, RJ;Norris, DE
通讯作者：
Norris, DE
影响因子：
3.3
作者：
Pinchoff, Jessie;Hamapumbu, Harry;Moss, William J.
通讯作者：
Moss, William J.
影响因子：
3.3
作者：
Parr, Jonathan B.;Belson, Connor;Juliano, Jonathan J.
通讯作者：
Juliano, Jonathan J.
影响因子：
14.9
作者：
Altschul, SF;Madden, TL;Lipman, DJ
通讯作者：
Lipman, DJ