Construction of Boolean logic gates based on dual-vector circuits of multiple gene regulatory elements
Construction of Boolean logic gates based on dual-vector circuits of multiple gene regulatory elements
复制标题
基于多基因调控元件双向量电路的布尔逻辑门构建
DOI：
10.1007/s00438-018-1502-x
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发表时间：
2019-04-01
影响因子：
3.1
通讯作者：
Xu，Donggang
中科院分区：
文献类型：
--
作者：
Wei，Zhao;Fu，Wenliang;Xu，Donggang
基因电路被构建为运行复杂的逻辑运算，以精确调节生物代谢过程。目前，大多数遗传电路的实现是基于各种电路元件的调节机制，但我们希望通过生物体的生物代谢途径来实现复杂的逻辑门。在这项研究中，我们匹配不同功能机制的调节元件，通过双向量电路来构建布尔逻辑门模型。在大肠杆菌中，我们制作了12个电路逻辑门模块，并通过一个报告基因的表达和分析验证了其中四个逻辑门的功能，包括“AND”、“NAND”、“OR”和“NOR”。寄主新陈代谢的中间产物将输入转化为输出。结果表明，这些逻辑门电路具有预期的效率和调节特性。我们的研究为设计基因电路和精确控制代谢途径提供了新的思路。
Gene circuits are constructed to run complex logical operations for the precise regulation of biological metabolic processes. At present, the implementation of most genetic circuits is based on the regulatory mechanism of various circuit components, but we hope to realize complex logic gates through biological metabolic pathways of organisms. In this study, we matched the regulatory elements of different functional mechanisms to build a Boolean logic gate model by means of a dual-vector circuit. InEscherichia coli, we made 12 circuit logic gate modules and validated the functions of four of the logic gates, including “AND”, “NAND”, “OR” and “NOR” by the expression and analysis of a reporter gene. The inputs were converted into outputs by an intermediate product of the host metabolism. The results indicated that these logic gate circuits had the expected efficacy and regulatory characteristics. Our study provides new ideas for designing genetic circuits and precisely controlling metabolic pathways.