Secretion of α-immunoreactive inhibin by human pre-embryos cultured in vitro
Secretion of α-immunoreactive inhibin by human pre-embryos cultured in vitro
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体外培养的人前胚胎分泌α-免疫反应抑制剂
DOI：
10.1093/oxfordjournals.humrep.a137687
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发表时间：
1992
影响因子：
6.1
通讯作者：
P. Zourlas
中科院分区：
文献类型：
--
作者：
I. Phocas;A. Sarandakou;D. Rizos;F. Dimitriadou;T. Mantzavinos;P. Zourlas
使用酶免疫测定试剂盒在培养基（补充有14%热灭活的人血清的Ham’s F-10培养基）中从受精后第3天或第4天至第14天测量来自参与体外受精程序的8名妇女的31个剩余前胚胎的α-免疫反应性的白细胞介素。从受精后第4天开始，所有的受精卵都表现出分泌抑制（平均值+/- SEM：3.0 +/- 0.7 U），并且与前胚的形态发育无关（2-4个细胞，n = 4; 6-8个细胞，n = 4; 8-10个细胞，n = 9; 10-12个细胞，n = 4;桑椹胚，n = 5和囊胚，n = 4）。在受精后第7、10、13和14天，未解体前胚的平均Eclubin值+/- SEM分别为：6.5 +/- 0.9 U、12.3 +/- 2.0 U、16.8 +/- 3.2 U和20.2 +/- 3.7 U;然而，当在受精后第10天和第13天观察到崩解时，Eclubin平均值分别为9.0 +/- 1.4 U和8.4 +/- 1.7 U。抑制素水平显着相关，人绒毛膜促性腺激素水平在同一培养基中，只有在第13天，而妊娠特异性β 1-糖蛋白的相关性发生在受精后第7天。总之，早期人类前胚胎分泌α-免疫反应性细胞滋养层形成之前。当发育继续时，这种分泌随着时间的推移而显著增加，而崩解之后是白藜芦醇释放速率的净下降。
alpha-Immunoreactive inhibin was measured using an enzyme immunoassay kit in the culture medium (Ham's F-10 medium supplemented with 14% heat-inactivated human serum) from day 3 or 4 to day 14 post-fertilization of 31 surplus pre-embryos from eight women participating in an in-vitro fertilization programme. Inhibition secretion was demonstrated in all of them from the fourth day after fertilization (mean +/- SEM: 3.0 +/- 0.7 U) and was independent of the morphological development of pre-embryos (2-4 cells, n = 4; 6-8 cells, n = 4; 8-10 cells, n = 9; 10-12 cells, n = 4; morulae, n = 5 and blastocysts, n = 4). On days 7, 10, 13 and 14 post-fertilization, mean inhibin values +/- SEM for non-disintegrated pre-embryos were respectively: 6.5 +/- 0.9 U, 12.3 +/- 2.0 U, 16.8 +/- 3.2 U and 20.2 +/- 3.7 U; however, when disintegration was noted on days 10 and 13 after fertilization, inhibin mean values were 9.0 +/- 1.4 U and 8.4 +/- 1.7 U respectively. Inhibin levels were significantly correlated with human chorionic gonadotrophin levels in the same culture media only on day 13, while correlation with pregnancy specific beta 1-glycoprotein occurred on day 7 post-fertilization. In conclusion, early human pre-embryos secrete alpha-immunoreactive inhibin before the cytotrophoblast is formed. This secretion increases significantly with time when development is continued, while disintegration is followed by a net decline in the rate of inhibin release.