Involvement of the Heat Shock Protein HtpG of Salmonella Typhimurium in Infection and Proliferation in Hosts.
Involvement of the Heat Shock Protein HtpG of Salmonella Typhimurium in Infection and Proliferation in Hosts.
复制标题
鼠伤寒沙门氏菌热激蛋白HtpG参与宿主感染和增殖
DOI：
10.3389/fcimb.2021.758898
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发表时间：
2021
影响因子：
5.7
通讯作者：
Zuo J
中科院分区：
文献类型：
--
作者：
Dong T;Wang W;Xia M;Liang S;Hu G;Ye H;Cao Q;Dong Z;Zhang C;Feng D;Zuo J
鼠伤寒沙门氏菌是感染人和动物胃肠道的常见病原体，引起宿主胃肠炎和伤寒。热休克蛋白（HtpG）作为分子伴侣参与细菌的各种细胞过程，特别是在环境压力下。然而，HtpG 与鼠伤寒沙门氏菌感染的潜在关联仍不清楚。在这项研究中，我们阐明了 HtpG 也可以在鼠伤寒沙门氏菌感染中发挥效应子的作用。 RNA-seq表明HtpG突变后鼠伤寒沙门氏菌鞭毛组装途径、感染途径和趋化途径基因下调，导致鼠伤寒沙门氏菌运动、生物膜形成、粘附、侵袭和炎症诱导能力受损。此外，HtpG重组蛋白能够促进鼠伤寒沙门氏菌在宿主细胞中的增殖以及由此产生的炎症。总的来说，我们的结果说明了 HtpG 在鼠伤寒沙门氏菌感染中的重要作用。
Salmonella Typhimurium is a common pathogen infecting the gastrointestinal tract of humans and animals, causing host gastroenteritis and typhoid fever. Heat shock protein (HtpG) as a molecular chaperone is involved in the various cellular processes of bacteria, especially under environmental stress. However, the potential association of HtpG with S. Typhimurium infection remains unknown. In this study, we clarified that HtpG could also play a role as an effector in S. Typhimurium infection. RNA-seq indicated that the flagellar assembly pathway, infection pathway, and chemotaxis pathway genes of S. Typhimurium were downregulated after the mutation of HtpG, which resulted in compromises of S. Typhimurium motility, biofilm formation, adhesion, invasion, and inflammation-inducing ability. In addition, HtpG recombinant protein was capable of promoting the proliferation of S. Typhimurium in host cells and the resultant inflammation. Collectively, our results illustrated an important role of HtpG in S. Typhimurium infection.
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DOI：
10.1126/science.1165771
发表时间：
2008-12-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Marraffini LA;Sontheimer EJ
通讯作者：
Sontheimer EJ
DOI：
10.1073/pnas.0913087107
发表时间：
2010-02-16
影响因子：
11.1
作者：
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通讯作者：
Aderem, Alan
DOI：
10.1073/pnas.1822018116
发表时间：
2019-06-18
影响因子：
11.1
作者：
Desai, Stuti K.;Padmanabhan, Anup;Kenney, Linda J.
通讯作者：
Kenney, Linda J.
影响因子：
30.5
作者：
Franchi, Luigi;Amer, Amal;Nunez, Gabriel
通讯作者：
Nunez, Gabriel
影响因子：
3.4
作者：
Boyle, Erin C.;Brown, Nat F.;Finlay, B. Brett
通讯作者：
Finlay, B. Brett