Archived Formalin-Fixed Paraffin-Embedded (FFPE) Blocks: A Valuable Underexploited Resource for Extraction of DNA, RNA, and Protein
Archived Formalin-Fixed Paraffin-Embedded (FFPE) Blocks: A Valuable Underexploited Resource for Extraction of DNA, RNA, and Protein
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DOI：
10.1089/bio.2012.0052
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发表时间：
2013-04-01
影响因子：
1.6
通讯作者：
Baloch, Zubair W.
中科院分区：
文献类型：
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作者：
Kokkat, Theresa J.;Patel, Miral S.;Baloch, Zubair W.
福尔马林固定石蜡包埋(FFPE)材料在各种生物标志物的研究中提供了一个现成的资源。储存期是否对所提取的大分子有显著影响一直是人们感兴趣的问题。因此，在本研究中，我们调查了储存时间是否对提取的核酸和蛋白质的数量/质量有影响。我们系统地检测了储存多年的肺癌、甲状腺和唾液腺恶性肿瘤的FFPE块中基因组DNA、总RNA和总蛋白的质量/数量。我们发现，从储存11-12年、5-7年或1-2年的区块中提取的大分子与当前年份区块相比没有显著差异。
Formalin-fixed paraffin-embedded (FFPE) material presents a readily available resource in the study of various biomarkers. There has been interest in whether the storage period has significant effect on the extracted macromolecules. Thus, in this study, we investigated if the storage period had an effect on the quantity/quality of the extracted nucleic acids and proteins. We systematically examined the quality/quantity of genomic DNA, total RNA, and total protein in the FFPE blocks of malignant tumors of lung, thyroid, and salivary gland that had been stored over several years. We show that there is no significant difference between macromolecules extracted from blocks stored over 11-12 years, 5-7 years, or 1-2 years in comparison to the current year blocks.