VqbZIP1 isolated from Chinese wild Vitis quinquangularis is involved in the ABA signaling pathway and regulates stilbene synthesis
VqbZIP1 isolated from Chinese wild Vitis quinquangularis is involved in the ABA signaling pathway and regulates stilbene synthesis
复制标题
从中国野生五角葡萄中分离的VqbZIP1参与ABA信号通路并调节二苯乙烯合成
DOI：
10.1016/j.plantsci.2019.110202
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发表时间：
2019
期刊：
影响因子：
5.2
通讯作者：
Yuejin Wang
中科院分区：
文献类型：
--
作者：
Dan Wang;Changyue Jiang;Ruimin Li;Yuejin Wang
白藜芦醇是葡萄藤重要的植物抗毒素。它不仅能增强抗病能力，而且作为一种食品成分，它对人类健康有显著的益处。脱落酸（阿坝）是一种重要的植物激素，参与植物体内的许多生物学过程，也能促进二苯乙烯类化合物的积累。芪合酶（STS）是白藜芦醇合成最后一步的重要催化酶。我们的研究特征的碱性亮氨酸拉链（bZIP）转录因子，VqbZIP 1，从中国野生葡萄登记丹凤2。结果表明，VqbZIP 1编码299个氨基酸，属于bZIP家族的A组亚家族。VqbZIP1在酵母中显示出转录激活活性，并被预测位于细胞核中。酵母双杂交实验和双分子荧光互补（BiFC）实验表明，VqbZIP1与VqSnRK2.4和VqSnRK2.6相互作用，阿坝处理可诱导VqbZIP1、STS基因、VqSnRK2.4和VqSnRK2.6的表达。GUS活性实验表明VqbZIP 1能够激活STS启动子驱动的GUS报告基因。进一步的研究表明，VqbZIP 1与VqSnRK2.4或VqSnRK2.6的共表达在激活STS启动子方面可以赋予比单独表达VqbZIP 1更高的效率。VqbZIP 1在葡萄叶片中的过表达促进了STS基因的转录水平和芪类化合物的积累。VqbZIP1在拟南芥中的过表达可赋予阿坝敏感性。综上所述，我们的研究结果表明，VqbZIP 1参与阿坝信号通路，并调节芪的合成。
Resveratrol is an important phytoalexin in grapevine. Not only does it confer increased disease resistance and but as a food component it offers significant benefits in human health. Abscisic acid (ABA) is an important phytohormone involved in many biological processes in plants and can also promote the accumulation of stilbenes. Stilbene synthase (STS) is an important enzyme which catalyzes the last step of resveratrol synthesis. Our study characterizes a basic leucine zipper (bZIP) transcription factor,VqbZIP1, isolated from Chinese wildVitis quinquangularisaccession Danfeng-2. The results show thatVqbZIP1encodes 299 amino acids and belongs to the Group A subfamily of the bZIP family. VqbZIP1 showed transcriptional activation activity in yeast and is predicted to be located in the nucleus. The yeast two-hybrid assay and bimolecular fluorescence complementation (BiFC) assay together show that VqbZIP1 interacts with VqSnRK2.4 and VqSnRK2.6.VqbZIP1, theSTSgenes,VqSnRK2.4andVqSnRK2.6can all be induced by ABA treatment. A GUS activity experiment indicatesVqbZIP1can activate the GUS reporter gene driven by STS promoters. Further studies show that co-expression ofVqbZIP1withVqSnRK2.4orVqSnRK2.6can confer higher efficiency than expression ofVqbZIP1alone in activating the STS promoters. Overexpression ofVqbZIP1in grape leaves promoted the transcript level of theSTSgenes and the accumulation of stilbenes. Overexpression ofVqbZIP1in Arabidopsis thaliana can confer ABA sensitivity. In summary, our results suggestVqbZIP1participates in the ABA signaling pathway and regulates stilbene synthesis.