Automating the application of smart materials for protein crystallization.
Automating the application of smart materials for protein crystallization.
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自动化智能材料在蛋白质结晶中的应用。
DOI：
10.1107/s1399004714027643
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Chayen NE
中科院分区：
文献类型：
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作者：
Khurshid S;Govada L;El-Sharif HF;Reddy SM;Chayen NE
描述了第一种用于自动蛋白质结晶试验的半液体非蛋白质成核剂。这种“智能材料”被证明可以诱导晶体生长，并将为学术界和工业界的科学家提供一种简单，具有成本效益的工具。报道了第一种半液体非蛋白质成核剂的制造和验证，该成核剂可自动用于结晶试验。这项研究建立在分子印迹聚合物（MIPs;称为“智能材料”）诱导蛋白质晶体生长的适用性的先前证明的基础上。经证明，适合于高通量试验的改变织构的改性MIP在筛选合适的结晶条件时改善晶体质量并增加成功的可能性。这些材料的应用是简单的，时间效率高，并将提供一个强有力的工具，结构生物学家着手结晶试验。
The first semi-liquid, non-protein nucleating agent for automated protein crystallization trials is described. This ‘smart material’ is demonstrated to induce crystal growth and will provide a simple, cost-effective tool for scientists in academia and industry. The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imprinted polymers (MIPs; known as ‘smart materials’) for inducing protein crystal growth. Modified MIPs of altered texture suitable for high-throughput trials are demonstrated to improve crystal quality and to increase the probability of success when screening for suitable crystallization conditions. The application of these materials is simple, time-efficient and will provide a potent tool for structural biologists embarking on crystallization trials.