Growth and metabolism of human hepatocytes on biomodified collagen poly(lactic-co-glycolic acid) three-dimensional scaffold
Growth and metabolism of human hepatocytes on biomodified collagen poly(lactic-co-glycolic acid) three-dimensional scaffold
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DOI：
10.1097/01.mat.0000217794.35830.4a
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发表时间：
2006-05-01
期刊：
影响因子：
4.2
通讯作者：
Gong, YH
中科院分区：
文献类型：
--
作者：
Li, J;Li, LJ;Gong, YH
肝组织工程为减轻供体肝的需求提供了一种很有前途的方法，但必须克服许多挑战，包括支架的选择，细胞来源和免疫屏障。聚（乳酸-乙醇酸）（PLGA）聚合物是一种创新的可生物降解材料，已被证明可用作接种和培养各种类型细胞的支架。在这项研究中，多孔海绵支架的改性PLGA聚合物与胶原蛋白的能力，以提高人肝细胞的生长和代谢进行了研究。我们评价了胶原修饰的PLGA（C-PLGA）支架与从人肝中分离的肝细胞的生物相容性。在不同的培养阶段评估细胞粘附和功能（细胞密度、培养寿命、白蛋白合成、尿素合成、氨消除和地西泮清除）。C-PLGA支架中培养的肝细胞数量高于未进行胶原修饰的PLGA支架中培养的肝细胞数量，并且C-PLGA支架中培养的肝细胞的寿命长于PLGA支架中培养的细胞。白蛋白和尿素的合成和氨从附着的肝细胞中消除C-PLGA比在PLGA支架，与地西泮清除例外。胶原改性PLGA支架是一种很有前途的肝组织工程生物材料。
Hepatic tissue engineering offers a promising approach toward alleviating the need for donor liver, yet many challenges must be overcome including choice of scaffold, cell source, and immunologic barriers. Poly(lactic-co-glycolic acid) (PLGA) polymers are innovative biodegradable materials that have been shown to be useful as scaffolds for seeding and culturing various types of cells. In this study, a porous sponge scaffold of modified PLGA polymer with collagen was investigated for its ability to improve the growth and metabolism of human hepatocytes. We evaluated the biocompatibility of collagen-modified PLGA (C-PLGA) scaffolds with hepatocytes isolated from human liver. Cell adhesion and function (cell density, culture lifespan, albumin synthesis, urea synthesis, and ammonia elimination and diazepam clearance) were assessed during different culture periods. The number of hepatocytes cultured in C-PLGA scaffolds was higher compared with those cultured in PLGA scaffolds without collagen modification, and the lifespan of hepatocytes cultured in C-PLGA scaffolds was longer than that of cells cultured in PLGA scaffolds. Albumin and urea synthesis and ammonia elimination from attached hepatocytes were greater in C-PLGA than in PLGA scaffolds, with the exception of diazepam clearance. Collagen-modified PLGA scaffold is a promising biomaterial for hepatic tissue engineering.