Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport.
Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport.
复制标题
自旋回波核磁共振在全细胞系统中的应用。
作者：
K. Brindle;F. F. Brown;I. Campbell;C. Grathwohl;P. Kuchel
提出了一种研究膜转运的新方法。高分辨率核磁共振成像用于测量小分子在细胞内和细胞外区室之间的分布。该方法使用自旋回波技术，并依赖于细胞内外介质磁化率的差异。它还提供了关于细胞代谢状态的同步信息。该方法说明了丙氨酸和乳酸盐在人红细胞中的运输的研究。
A new method for studying membrane transport is presented. High resolution n.m.r. is used to measure the distribution of small molecules between the intracellular and extracellular compartments. The method uses spin-echo techniques and relies on a difference in the magnetic susceptibility of the media inside and outside of cells. It also provides simultaneous information on the metabolic status of the cell. The method is illustrated by a study of alanine and lactate transport in the human erythrocyte.