Stochasticity constrained by deterministic effects of diet and age drive rumen microbiome assembly dynamics
Stochasticity constrained by deterministic effects of diet and age drive rumen microbiome assembly dynamics
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DOI：
10.1038/s41467-020-15652-8
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发表时间：
2020-04-20
影响因子：
16.6
通讯作者：
Mizrahi, Itzhak
中科院分区：
文献类型：
--
作者：
Furman, Ori;Shenhav, Liat;Mizrahi, Itzhak
复杂的群落如何在动物的一生中聚集，以及这个过程的可预测性如何，仍然没有被探索。在这里，我们调查的力量，推动整个奶牛的生活瘤胃微生物组的组装，强调随机和确定性过程之间的平衡。我们使用16 S-rRNA扩增子测序数据分析了从出生到成年的瘤胃微生物组的发育，发现这些动物共享一组核心演替物种，这些物种在早期入侵并持续到成年。沿着确定性因素，如年龄和饮食，早期到达的物种产生了强烈的优先效应，晚期演替类群的动态强烈依赖于早期生命阶段的微生物组组成。优先效应还表现为剖腹产与自然分娩的动物之间微生物组发育动态的巨大变化，前者经历了更快的物种入侵和加速的微生物组发育。总的来说，我们的研究结果表明，加上饮食和年龄所施加的强确定性约束，早期生命中的随机定植对动物微生物组的发育具有长期影响。复杂的微生物群落如何在动物的一生中组装，以及组装过程的可预测性如何，人们仍然知之甚少。在这里，作者描述了剖腹产或自然分娩的动物从出生到成年的牛肠道微生物组，并表明生命早期的偶然事件对微生物组发育有很大影响。
How complex communities assemble through the animal's life, and how predictable the process is remains unexplored. Here, we investigate the forces that drive the assembly of rumen microbiomes throughout a cow's life, with emphasis on the balance between stochastic and deterministic processes. We analyse the development of the rumen microbiome from birth to adulthood using 16S-rRNA amplicon sequencing data and find that the animals shared a group of core successional species that invaded early on and persisted until adulthood. Along with deterministic factors, such as age and diet, early arriving species exerted strong priority effects, whereby dynamics of late successional taxa were strongly dependent on microbiome composition at early life stages. Priority effects also manifest as dramatic changes in microbiome development dynamics between animals delivered by C-section vs. natural birth, with the former undergoing much more rapid species invasion and accelerated microbiome development. Overall, our findings show that together with strong deterministic constrains imposed by diet and age, stochastic colonization in early life has long-lasting impacts on the development of animal microbiomes. How complex microbial communities assemble through the animal's life, and how predictable the assembly process is, remains poorly understood. Here, the authors profile the cow gut microbiome from birth to adulthood in animals born in C-section or natural birth and show that chance events early in life have a strong impact on microbiome development.