The gain-of-function mutation blf13 in the barley orthologue of the rice growth regulator NARROW LEAF1 is associated with increased leaf width
The gain-of-function mutation blf13 in the barley orthologue of the rice growth regulator NARROW LEAF1 is associated with increased leaf width
复制标题
水稻生长调节剂 NARROW LEAF1 的大麦直系同源物中的功能获得突变 blf13 与叶宽增加相关
DOI：
10.1093/jxb/erad403
复制
发表时间：
2024
影响因子：
6.9
通讯作者：
Jöst M
中科院分区：
文献类型：
--
作者：
Jöst M
谷类作物的冠层结构在决定产量方面起着重要作用。树叶宽度代表了这个树冠结构的一个关键方面。然而，我们对谷类作物叶宽控制的理解仍然不完整。经典的突变研究几乎没有发现多个形态突变，包括那些具有不同叶宽的突变。其中，我们对宽叶13(Blf13)突变体进行了详细的鉴定。突变植株由于启动后生长和细胞增殖增加而形成更宽的叶片。突变表型与HvHNT1基因中的一个错义突变完美地共分离，该错义突变影响到编码蛋白的一个高度保守的区域，该区域与水稻狭窄LEAF1(NAL1)蛋白同源。相关的转录分析和蛋白质-蛋白质相互作用研究进一步支持了blf13表型的这种突变的因果关系，这些研究表明，突变的HvNHT1蛋白与已知的相互作用因子的相互作用比野生型HvHNT1更强。与野生型HvHNT1相比，突变型HvHNT1蛋白也表现出更强的同源二聚化，同源模拟表明由于blf13突变，HvHNT1单体之间增加了一个相互作用位点。因此，blf13突变与水稻中增加叶片宽度和籽粒产量的已知功能增益NAL1等位基因平行，这表明blf13突变在大麦中可能具有类似的农学潜力。
Canopy architecture in cereals plays an important role in determining yield. Leaf width represents one key aspect of this canopy architecture. However, our understanding of leaf width control in cereals remains incomplete. Classical mutagenesis studies in barely identified multiple morphological mutants, including those with differing leaf widths. Of these, we characterized thebroad leaf13(blf13) mutant in detail. Mutant plants form wider leaves due to increased post-initiation growth and cell proliferation. The mutant phenotype perfectly co-segregated with a missense mutation in theHvHNT1gene which affected a highly conserved region of the encoded protein, orthologous to the rice NARROW LEAF1 (NAL1) protein. Causality of this mutation for theblf13phenotype is further supported by correlative transcriptomic analyses and protein–protein interaction studies showing that the mutant HvNHT1 protein interacts more strongly with a known interactor than wild-type HvHNT1. The mutant HvHNT1 protein also showed stronger homodimerization compared with wild-type HvHNT1, and homology modelling suggested an additional interaction site between HvHNT1 monomers due to theblf13mutation. Thus, theblf13mutation parallels known gain-of-functionNAL1alleles in rice that increase leaf width and grain yield, suggesting that theblf13mutation may have a similar agronomic potential in barley.