Effect of therapeutic pulsed ultrasound on parameters of oxidative stress in skeletal muscle after injury
Effect of therapeutic pulsed ultrasound on parameters of oxidative stress in skeletal muscle after injury
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DOI：
10.1016/j.cellbi.2006.11.015
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发表时间：
2007-05-01
影响因子：
3.9
通讯作者：
Streck, Emilio L.
中科院分区：
文献类型：
--
作者：
Freitas, Luciana S.;Freitas, Tiago P.;Streck, Emilio L.
挫伤是运动和非运动损伤的一种非常常见的形式，会影响肌肉功能。增强正常修复和再生过程的治疗对于各种患者都很重要。据称，治疗性超声波可以促进组织修复，特别是通过增强细胞增殖和蛋白质合成。本研究旨在探讨治疗性脉冲超声（TPU）对损伤后骨骼肌氧化应激参数的影响，即硫代巴比妥酸反应物质（TBARS）、蛋白质羰基含量以及抗氧化酶、过氧化氢酶和超氧化物歧化酶（SOD）的活性。将 Wistar 大鼠置于肌肉（腓肠肌）撕裂动物模型中。 TPU每天使用一次。肌肉撕裂后一、三或五天，通过断头处死动物并评估氧化应激参数。肌肉损伤动物血清CK水平升高，表明撕裂伤动物模型成功。与假手术组相比，肌肉损伤后 TBARS 没有改变。肌肉撕裂后蛋白质羰基含量增加。过氧化氢酶和 SOD 活性在肌肉损伤后 1 天增加，并且在第 3 天和第 5 天没有改变。与未经治疗的受伤肌肉动物相比，TPU 降低了肌肉撕裂后的 TBARS 水平。蛋白质羰基含量评估呈现相似的结果。人们很容易推测 TPU 似乎可以保护组织免受氧化损伤。 TPU 降低了过氧化氢酶和 SOD 活性，尤其是在肌肉撕裂后的第一天。 (C) 2006 年国际细胞生物学联合会。由爱思唯尔有限公司出版。保留所有权利。
Contusion injuries are a very common form of both athletic and non-athletic injury, that effect muscle function. Treatments to augment the normal repair and regeneration processes are important for a wide variety of patients. Therapeutic ultrasound has been claimed to promote tissue repair, especially by enhancing cell proliferation and protein synthesis. The present study aimed to investigate the effect of therapeutic pulsed ultrasound (TPU) on parameters of oxidative stress, namely thiobarbituric acid-reactive substances (TBARS), protein carbonyl content and the activities of antioxidant enzymes, catalase and superoxide dismutase (SOD), in skeletal muscle after injury. Wistar rats were submitted to an animal model of muscle (gastrocnemius) laceration. TPU was used once a day. One, three or five days after muscle laceration, the animals were killed by decapitation and oxidative stress parameters were evaluated. Serum CK levels were increased in muscle-injured animals, indicating that the laceration animal model was successful. TBARS were not altered after muscle injury, when compared to the sham group. Protein carbonyl content was increased after muscle laceration. Catalase and SOD activities were increased 1 day after muscle injury and not altered at days 3 and 5. TPU decreased TBARS levels after muscle laceration when compared to injured muscle animals without treatment. Protein carbonyl content evaluation presented similar results. It is tempting to speculate that TPU seems to protect the tissue from oxidative injury. TPU diminished catalase and SOD activities, especially on the first day following muscle laceration. (C) 2006 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.