The expression of matrix metalloproteinase matrilysin indicates the degree of inflammation in ulcerative colitis
The expression of matrix metalloproteinase matrilysin indicates the degree of inflammation in ulcerative colitis
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DOI：
10.1007/s005350300062
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发表时间：
2003-04-01
影响因子：
6.3
通讯作者：
Imai, K
中科院分区：
文献类型：
--
作者：
Matsuno, K;Adachi, Y;Imai, K
背景细胞外基质的合成和分解的任何改变在炎症和伤口愈合期间的组织重塑中是重要的。细胞外基质成分的降解受基质金属蛋白酶（MMP）级联反应的调节。本研究试图评估基质金属蛋白酶与溃疡性结肠炎炎症程度之间的关系。方法.采用免疫组化法检测了52例溃疡性结肠炎患者活检或手术切除的石蜡包埋组织中MMP-1、MMP-2、MMP-3、MMP-7（matrilysin）、MMP-9及其抑制剂TIMP-1、TIMP-2的表达。结果观察到MMP-1、-2、-9和TIMP在基质细胞中表达，MMP-3在上皮细胞和基质成分中都表达，而基质溶解素仅在溃疡边缘的上皮细胞中表达。与TIMPs相比，MMPs的表达增强。基质溶解素表达的频率相应于炎症的严重程度而增加。基质溶素也表达在上皮细胞与异型增生和癌症。结论.基质溶素的表达可能是一个重要的活性标志物，并可用于预测溃疡性结肠炎患者随后的转化。
Background. Any alteration in the synthesis and breakdown of the extracellular matrix is important in tissue remodeling during inflammation and wound healing. The degradation of the extracellular matrix components is regulated by a cascade of matrix metalloproteinases (MMPs). The present study attempted to assess the relationship between MMPs and the degree of inflammation in ulcerative colitis. Methods. The expression of MMPs, including MMP-1, -2, -3, -7 (matrilysin), and -9, and that of their inhibitors (tissue inhibitor of metalloproteinases [TIMP]-1 and -2) were analyzed immunohistochemically by using 52 formalin-fixed and paraffin-embedded specimens from patients with ulcerative colitis who had undergone a biopsy or surgery. Results. It was observed that MMP-1, -2, and -9, and the TIMPs were expressed in stromal cells, MMP-3 was expressed in both the epithelial cells and stromal components, and matrilysin was expressed only in the epithelial cells on the edge of ulcers. The expression of the MMPs was increased compared with that of the TIMPs. The frequency of matrilysin expression was increased corresponding to the severity of the inflammation. Matrilysin was also expressed in epithelial cells with dysplasia and cancer. Conclusions. Matrilysin expression could be an important marker of activity and could be used for the prediction of subsequent transformation in patients with ulcerative colitis.