No evidence for involvement of the promoter polymorphism -: 866 G/A of the UCP2 gene in childhood-onset obesity in humans
No evidence for involvement of the promoter polymorphism -: 866 G/A of the UCP2 gene in childhood-onset obesity in humans
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DOI：
10.1055/s-2003-39232
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发表时间：
2003-04-01
影响因子：
1.8
通讯作者：
Hebebrand, J
中科院分区：
文献类型：
--
作者：
Schäuble, N;Geller, F;Hebebrand, J
近年来，解偶联蛋白2基因启动子区-866 G/A多态性的G等位基因与肥胖相关。该多态性的等位基因频率和基因型分布在肥胖个体和从不肥胖的对照组之间存在差异。采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）进行基因分型，应用家系不平衡检验分析了93个同胞对家系中200名极度肥胖儿童和青少年G等位基因（野生型）的传递不平衡。此外，使用单侧渐近Pearson卡方检验，我们检验了G等位基因在277名极度肥胖儿童和青少年（包括93名一致同胞对的指数患者）中的出现频率是否高于188名从未肥胖的对照。单侧渐近Cochran Armitage趋势检验用于确定极度肥胖和健康体重不足个体之间的基因型频率差异。PDT分析显示，没有证据表明肥胖的传播不平衡。等位基因和基因型频率在极度肥胖和从不肥胖的受试者之间没有差异。总之，我们无法证实Esterbauer等人（2001）在我们年轻样本中的结果。
Recently, an association between obesity and the G-allele of the - 866 G/A polymorphism in the promoter region of uncoupling protein-2 gene (UCP2) was reported. Both allele frequencies and genotype distributions for this polymorphism differed between obese individuals and never-obese controls.We attempted to confirm this finding. Genotyping was performed by polymerase chain reaction with subsequent restriction fragment length polymorphism analysis (PCR-RFLP).We analysed transmission disequilibrium of the (wild type) G-allele for 200 extremely obese children and adolescents from 93 concordant sib pair families using the pedigree disequilibrium test. Additionally, using a one-sided asymptotic Pearson's chi(2)-test, we tested whether the G-allele occurs more frequently in 277 extremely obese children and adolescents (including the 93 index patients of the concordant sib pairs) than in 188 never-obese controls. The one-sided asymptotic Cochran Armitage trend test was used to determine differences in genotype frequencies between extremely obese and healthy underweight individuals. The PDT analysis revealed no evidence for transmission disequilibrium in obesity. Allele and genotype frequencies did not differ between the extremely obese and never-obese subjects. In conclusion, we cannot confirm the results of Esterbauer et al. (2001) in our young sample.