Structure-Function Relationships of Anthocyanins from Various Anthocyanin-Rich Extracts on the Inhibition of Colon Cancer Cell Growth
Structure-Function Relationships of Anthocyanins from Various Anthocyanin-Rich Extracts on the Inhibition of Colon Cancer Cell Growth
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DOI：
10.1021/jf8005917
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发表时间：
2008-10-22
影响因子：
6.1
通讯作者：
Giusti, M. Monica
中科院分区：
文献类型：
--
作者：
Jing, Pu;Bomser, Joshua A.;Giusti, M. Monica
花青素是一种有效的抗氧化剂，可能具有化学保护作用。然而，结构与功能的关系还没有被很好地理解。本研究的目的是比较不同花色苷类型的富含花色苷提取物(ARs)的化学保护性能，以了解花色苷化学结构与化学保护活性之间的关系，以抑制结肠癌细胞的增殖。此外，还研究了花色苷与其他酚类化合物之间的化学保护作用。用人结肠腺癌(HT29)细胞株检测了不同花色苷谱的紫玉米、木莓、越橘、紫胡萝卜、葡萄、萝卜和接骨木的生长抑制作用(GI(50))。Ares对HT29细胞的生长均有不同程度的抑制作用，表现为：紫玉米(GI(50)相当于14mgCy-3-Glu当量/毫升)、紫胡萝卜、葡萄、紫胡萝卜、接骨木(GI(50)、100mgCy-3-Glu当量/mL)。花色苷在白藜芦醇的化学保护中起主要作用，并与存在的其他酚类物质产生相加作用。统计分析表明，花色苷的化学结构影响化学保护，其中未经酰化的单糖化花色苷对HT-29细胞的增殖抑制作用较大，而含有天葵素、三糖苷和/或与肉桂酸的酰化作用的花色苷的抑制作用最小。这些发现应该被考虑用于作物选择和富含花青素的功能食品的开发。
Anthocyanins are potent antioxidants and may be chemoprotective. However, the structure-function relationships are not well understood. The objectives of this study were to compare the chemoprotective properties of anthocyanin-rich extracts (AREs) with variable anthocyanin profiles to understand the relationship between anthocyanin chemical structure and chemoprotective activity, measured as inhibition of colon cancer cell proliferation. Additionally, the chemoprotective interaction of anthocyanins and other phenolics was investigated. AREs with different anthocyanin profiles from purple corn, chokeberry, bilberry, purple carrot, grape, radish, and elderberry were tested for growth inhibition (GI(50)) using a human colorectal adenocarcinoma (HT29) cell line. All AREs suppressed HT29 cell growth to various degrees as follows: purple corn (GI(50) similar to 14 mu g of cy-3-glu equiv/mL) > chokeberry and bilberry > purple carrot and grape > radish and elderberry (GI(50) > 100 mu g of cy-3-glu equiv/mL). Anthocyanins played a major role in AREs' chemoprotection and exerted an additive interaction with the other phenolics present. Statistical analyses suggested that anthocyanin chemical structure affected chemoprotection, with nonacylated monoglycosylated anthocyanins having greater inhibitory effect on HT-29 cell proliferation, whereas anthocyanins with pelargonidin, triglycoside, and/or acylation with cinnamic acid exerted the least effect. These findings should be considered for crop selection and the development of anthocyanin-rich functional foods.