Temporal and spatial diversities of theimmunolabeling of mannan and xylanpolysaccharides in differentiatingearlywood ray cells and pits of Cryptomeria japonica
Temporal and spatial diversities of theimmunolabeling of mannan and xylanpolysaccharides in differentiatingearlywood ray cells and pits of Cryptomeria japonica
复制标题
甘露聚糖和木聚糖多糖免疫标记在区分柳杉早材射线细胞和纹核中的时空多样性
DOI：
10.1007/s00425-010-1283-x
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发表时间：
2011
期刊：
影响因子：
4.3
通讯作者：
Arata Yoshinaga and Keiji Takabe
中科院分区：
文献类型：
--
作者：
Jong Sik Kim;Tatsuya Awano;Arata Yoshinaga and Keiji Takabe
木材由各种类型的细胞组成，每种类型的细胞具有不同的结构和功能特性。然而，细胞壁组分在不同细胞类型之间的时间和空间差异很少被理解。为了扩展我们对细胞壁组分在细胞间分布差异的理解，我们研究了射线细胞和纹孔中甘露聚糖（O-乙酰基-半乳糖葡甘露聚糖，GGM）和木聚糖（阿拉伯-4-O-甲基葡糖醛酸聚糖，AGX）的免疫标记。GGM和AGXs的标记在射线细胞中存在时间差异。GGM标记在管胞S1形成早期的射线细胞中开始检测到，而AGX标记在管胞S2形成期的射线细胞中开始检测到。射线细胞中GGM和AGX标记的发生与管胞中GGM和AGX标记的发生也存在时间上的差异。AGX标记开始检测射线细胞比管胞晚得多。射线细胞的GGM标记也比管胞稍早或稍晚。在纹孔中，在纹孔形成的早期阶段，在边缘和交叉场纹孔膜中检测到GGM标记，但在成熟的纹孔中没有观察到，这表明能够降解GGM的酶可能参与纹孔膜的形成。与GGM相反，在整个发育过程中，在有边界和交叉领域的坑膜中没有检测到AGX。AGXs显示出结构和沉积的变化，在坑边界取决于发展阶段的边界和跨领域的坑。
Wood is composed of various types of cells and each type of cell has different structural and functional properties. However, the temporal and spatial diversities of cell wall components in the cell wall between different cell types are rarely understood. To extend our understanding of distributional diversities of cell wall components among cells, we investigated the immunolabeling of mannans (O-acetyl-galactoglucomannans, GGMs) and xylans (arabino-4-O-methylglucuronoxylans, AGXs) in ray cells and pits. The labeling of GGMs and AGXs was temporally different in ray cells. GGM labeling began to be detected in ray cells at early stages of S1formation in tracheids, whereas AGX labeling began to be detected in ray cells at the S2formation stage in tracheids. The occurrence of GGM and AGX labeling in ray cells was also temporally different from that of tracheids. AGX labeling began to be detected much later in ray cells than in tracheids. GGM labeling also began to be detected in ray cells either slightly earlier or later than in tracheids. In pits, GGM labeling was detected in bordered and cross-field pit membranes at early stages of pit formation, but not observed in mature pits, indicating that enzymes capable of GGM degradation may be involved in pit membrane formation. In contrast to GGMs, AGXs were not detected in pit membranes during the entire developmental process of bordered and cross-field pits. AGXs showed structural and depositional variations in pit borders depending on the developmental stage of bordered and cross-field pits.