Inducing theta oscillations in the entorhinal hippocampal network in vitro
Inducing theta oscillations in the entorhinal hippocampal network in vitro
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DOI：
10.1007/s00429-016-1256-3
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发表时间：
2017-03-01
影响因子：
3.1
通讯作者：
Yakel, Jerrel L.
中科院分区：
文献类型：
--
作者：
Gu, Zhenglin;Yakel, Jerrel L.
海马θ节律是在主动探索过程中海马和海马相关大脑区域之间有节奏和同步的活动而出现的，为区域间交流提供了潜在的手段。然而，经过数十年的研究，theta 节律的起源仍然难以捉摸，至少部分原因是难以记录 theta 产生的所有三个重要区域，即海马体本身、隔膜和内嗅皮层。为此，我们通过将间隔胆碱能输入与海马局部活动配对，在间隔-内嗅-海马脑切片三培养系统中建立了体外 theta 模型。我们的研究表明，局部内嗅皮质回路可能在海马θ节律的产生中发挥积极和关键的作用。我们的研究还揭示了在缺乏明确起搏器的情况下，θ节律作为隔膜、海马和内嗅皮层之间动态相互作用的功能结果而出现的潜在机制。
The hippocampal theta rhythm emerges as rhythmic and synchronized activities among the hippocampus and hippocampus-associated brain regions during active exploration, providing a potential means for inter-regional communication. However, after decades of research, the origins of the theta rhythm remain elusive, at least partly due to the difficulty in recording from all three essential regions for theta generation, namely the hippocampus itself, the septum, and the entorhinal cortex. For this reason, we established an in vitro theta model in a septo-entorhinal-hippocampal brain slice tri-culture system by pairing septal cholinergic inputs with hippocampal local activities. Our study shows that the local entorhinal cortical circuit may play an active and critical role in hippocampal theta rhythm generation. Our study also reveals a potential mechanism for theta rhythms to emerge as the functional results of dynamic interactions among the septum, hippocampus, and the entorhinal cortex, in the absence of clear pace makers.