Visualization by comprehensive microarray analysis of gene expression programs during transdifferentiation of mesophyll cells into xylem cells
Visualization by comprehensive microarray analysis of gene expression programs during transdifferentiation of mesophyll cells into xylem cells
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DOI：
10.1073/pnas.232590499
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发表时间：
2002-11-26
影响因子：
11.1
通讯作者：
Fukuda, H
中科院分区：
文献类型：
--
作者：
Demura, T;Tashiro, G;Fukuda, H
植物有一种独特的转分化机制，分化的细胞可以通过这种机制启动新的分化程序。本研究对百日草(Zinnia elegans L.)的基因表达进行了综合分析。培养模型系统，以收集有关植物细胞转分化的基因调控的基本信息。在这个模型中，从百日葵叶片分离的光合叶肉细胞在形态发生过程中转分化为木质部细胞，其特征是次生壁形成和细胞程序性死亡。从培养细胞转分化为木质部细胞制备的均衡化的cDNA文库中分离到8000多个百日草的cDNA克隆。利用这些基因芯片分析揭示了几种独特的基因调控模式，包括：在细胞分离过程中一组基因的瞬时表达，推测是由创伤诱导的；在第一阶段，光合作用基因的表达迅速减少，并迅速诱导与蛋白质合成相关的基因；在随后的阶段，生长素相关基因的优先诱导；以及在最后阶段，与特定形态发生事件密切相关的基因的瞬时诱导，包括细胞壁的形成和降解以及细胞的程序性死亡。这项分析还揭示了一些以前未描述的编码在信号转导中起作用的蛋白质的基因，例如以阶段特异性方式表达的蛋白激酶和转录因子。这些发现为植物转分化和木材形成的分子机制提供了新的线索。
Plants have a unique transdifferentiation mechanism by which differentiated cells can initiate a new program of differentiation. We used a comprehensive analysis of gene expression in an in vitro zinnia (Zinnia elegans L.) culture model system to gather fundamental information about the gene regulation underlying the transdifferentiation of plant cells. In this model, photosynthetic mesophyll cells isolated from zinnia leaves transdifferentiate into xylem cells in a morphogenic process characterized by features such as secondary-wall formation and programmed cell death. More than 8,000 zinnia cDNA clones were isolated from an equalized cDNA library prepared from cultured cells transdifferentiating into xylem cells. Microarray analysis using these cDNAs revealed several types of unique gene regulation patterns, including: the transient expression of a set of genes during cell isolation, presumably induced by wounding; a rapid reduction in the expression of photosynthetic genes and the rapid induction of protein synthesis-associated genes during the first stage; the preferential induction of auxin-related genes during the subsequent stage; and the transient induction of genes closely associated with particular morphogenetic events, including cell-wall formation and degradation and programmed cell death during the final stage. This analysis also revealed a number of previously uncharacterized genes encoding proteins that function in signal transduction, such as protein kinases and transcription factors that are expressed in a stage-specific manner. These findings provide new clues to the molecular mechanisms of both plant transdifferentiation and wood formation.