Small Cell Carcinomas of the Uterine Cervix and Lung: Proteomics Reveals Similar Protein Expression Profiles.
Small Cell Carcinomas of the Uterine Cervix and Lung: Proteomics Reveals Similar Protein Expression Profiles.
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子宫颈和肺的小细胞癌：蛋白质组学揭示了相似的蛋白质表达谱。
DOI：
10.1097/igc.0000000000001354
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发表时间：
2018-11
期刊：
影响因子：
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通讯作者：
Kimura T
中科院分区：
文献类型：
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作者：
Egawa-Takata T;Yoshino K;Hiramatsu K;Nakagawa S;Serada S;Nakajima A;Endo H;Kubota S;Matsuzaki S;Kobayashi E;Ueda Y;Morii E;Inoue M;Naka T;Kimura T
小细胞宫颈癌（SMCC）和小细胞肺癌（SCLC）的表型和病理特征非常相似;因此，用于罕见SMCC的化疗方案通常基于用于常见SCLC的方案。我们着手探索这两种癌症之间的蛋白质表达谱相似性，以证明将其治疗方案联系起来是合理的，次要目的是找到肿瘤特异性蛋白质，用作更准确诊断SMCC的额外生物标志物，并可能用作治疗靶点。对3例SMCC和1例SCLC、1例宫颈粘液腺癌（MACC）、1例肺粘液腺癌（MACL）和1例宫颈鳞状细胞癌（SCC）进行蛋白表达分析。我们使用癌组织来源的球状体（CTOS）和同量异位素标签进行基于相对和绝对定量（iTRAQ）的全面和定量蛋白质表达谱分析。通过免疫组织化学方法验证相应临床样本中的表达。SMCC和SCLC样本显示了非常相似的蛋白表达谱，而不是器官来源特异性模式，这与它们匹配的肿瘤病理表型一致。16种蛋白质在小细胞癌（SMCC和SCLC）中的表达至少是MACC或SCC的2倍。免疫组化分析证实SMCC中肌酸激酶B型的表达高于MACC和SCC。我们证明了一个显着的重叠相似性的蛋白质表达谱的肺和宫颈小细胞癌，尽管显着差异，在其起源器官。
The phenotypic and pathological features of small cell cervical carcinoma (SMCC) and small small cell lung cancer (SCLC) are very similar; thus, the chemotherapy regimens used for the rare SMCC have been routinely based on regimens used for common SCLC. We set out to explore the protein expression profile similarities between these 2 cancers to prove that linking their therapeutic regimens is justified, with a secondary aim of finding tumor-specific proteins to use as additional biomarkers for more accurate diagnosis of SMCC, and potentially to use as therapeutic targets. Protein expression analysis was performed for 3 cases of SMCC and 1 example each of SCLC, mucinous adenocarcinoma of the cervix (MACC), lung mucinous adenocarcinoma (MACL), and squamous cell carcinoma of the cervix (SCC). We used cancer tissue–originated spheroids (CTOS) and isobaric tags for relative and absolute quantitation (iTRAQ)–based comprehensive and quantitative protein expression profile analysis. Expression in corresponding clinical samples was verified by immunohistochemistry. Rather than organ of origin–specific patterns, the SMCC and SCLC samples revealed remarkably similar protein expression profiles—in agreement with their matching tumor pathology phenotypes. Sixteen proteins were expressed at least 2-fold higher in both small cell carcinomas (SMCC and SCLC) than in MACC or SCC. Immunohistochemical analysis confirmed higher expression of creatine kinase B-type in SMCC, compared with MACC and SCC. We demonstrate a significant overlapping similarity of protein expression profiles of lung and cervical small cell carcinomas despite the significant differences in their organs of origin.