Sequence dependence of the B to Z transition in crystals and aqueous NaCl solutions for deoxyoligonucleotides containing all four canonical DNA bases.
Sequence dependence of the B to Z transition in crystals and aqueous NaCl solutions for deoxyoligonucleotides containing all four canonical DNA bases.
复制标题
含有所有四种典型 DNA 碱基的脱氧寡核苷酸的晶体和 NaCl 水溶液中 B 到 Z 转变的序列依赖性。
DOI：
10.1021/bi00390a042
复制
发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Peticolas,WL
中科院分区：
文献类型：
--
作者：
Wang,Y;Thomas,GA;Peticolas,WL
俄勒冈州尤金市俄勒冈大学化学系和分子生物学研究所，1986年11月20日收到；1987年3月17日收到修订稿件摘要：为了确定在高盐浓度(4-6M氯化钠)水溶液中哪些序列将以Z形结晶，哪些序列将在水溶液中进入Z形，对一系列包含所有四个典型脱氧核苷酸碱基[鸟嘌呤(G)、胞嘧啶(C)、腺嘌呤(A)和胸腺嘧啶(T)]的自互补八聚体脱氧核苷酸的构象进行了激光拉曼研究。D(TGCGCGCA)(I)、d(CACGCGTG)(II)、d(CGTGCACG)(III)和d(CGCATGCG)(IV)都是从低盐溶液中结晶出来的。微晶的拉曼光谱表明，I、II和IV以严格的Z形结晶，而III以B形结晶。序列I和II在0℃的4-6 M的氯化钠溶液中变为Z形，而序列III和IV在6 M的盐中保持B形。在晶体和高盐溶液中发现的Z型寡核苷酸的拉曼光谱有很大的差异。Z型在0℃的6 M氯化钠溶液中的拉曼光谱不是晶体中完整的Z型拉曼光谱和低盐溶液中完全的B型拉曼光谱的线性组合。这些低聚物的末端残基似乎不是严格的Z形。详细分析了所有残基在溶液和晶体中的环状折叠和正反构象。根据这些数据和最近文献中发现的数据，提出了一些简单的规则，这些规则可能有助于预测包含所有四个正则碱基的DNA序列在高盐条件下在水溶液中将进入Z形。这四个序列进入Z型的趋势可能是排序I&>II&>IV&>III。
Department of Chemistry and Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 Received November 20, 1986; Revised Manuscript Received March 17, 1987 abstract: A laser Raman study has been made on the conformation of a series of self-complementary octameric deoxynucleotides that contain all four canonical deoxynucleotide bases [guanine (G), cytosine (C), adenine (A), and thymine (T)] in order to determine which sequences will crystallize in the Z form and which sequences will go into the Z form in aqueous solution at high salt concentrations (4-6 M NaCl). All four octadeoxynucleotides, d (TGCGCGCA)(I), d (CACGCGTG)(II), d (CGTGCACG)(III), and d (CGCATGCG)(IV), have been crystallized from low-salt solutions. The Raman spectra of microcrystals show that I, II, and IV crystallize in a rigorous Z form while III crystallizes in the B form. Sequences I and II go into a Z form in 4-6 M NaCl solution at 0 C while sequences III and IV remain in the B form in 6 M salt. There are substantial differences in the Raman spectra of oligonucleotides in the Z form found in the crystal and in high-salt solutions. The Raman spectra of the Z forms in 6 M NaCl solution at 0 C are not linear combinations of the Raman spectra of the complete Z form in the crystal and the complete B form in low-salt solutions. The terminal residues of these oligomers do not appear to be in a strict Z form. A detailed analysis of the ring puckers and syn/anti conformation for all of the residues both in solution and in the crystal has been made. From these data together with data found in recent literature, some simple rules are suggested that may prove useful for predicting which DNA sequences containing all four canonical bases will go into the Z form in aqueous solution under high-salt conditions. The tendency of these four sequences to go into the Zform may be ranked I> II> IV> III.