Immunohistochemical study of interleukin-1beta and interleukin-1 receptor antagonist in an antigen-induced arthritis of the rabbit temporomandibular joint.
Immunohistochemical study of interleukin-1beta and interleukin-1 receptor antagonist in an antigen-induced arthritis of the rabbit temporomandibular joint.
复制标题
白细胞介素 1β 和白细胞介素 1 受体拮抗剂在抗原诱导的兔颞下颌关节关节炎中的免疫组织化学研究。
DOI：
--
复制
发表时间：
2002
影响因子：
3.3
通讯作者：
J. Fukuda
中科院分区：
文献类型：
--
作者：
M. Habu;K. Tominaga;M. Sukedai;P. Alstergren;S. Ohkawara;S. Kopp;J. Fukuda
背景
在颞下颌关节(TMJ)关节炎中，对IL-1β(IL-1β)和IL-1受体拮抗剂(IL-1ra)之间的关系以及这些细胞因子的来源知之甚少。我们用免疫组织化学方法研究了兔TMJ抗原性关节炎的发生发展过程。
方法
成年新西兰大白兔32只，建立单侧TMJ关节炎模型。造模后6h~12周，观察IL-1β和IL-1ra的变化。
结果
诱导后的关节炎急性期持续1周，之后转为慢性。在急性期早期，浸润性炎症细胞和滑膜细胞产生IL-1β和IL-1ra。在急性期晚期，这些细胞因子的主要来源是滑膜下成纤维细胞。在关节炎的这个阶段，IL-1β和IL-1ra似乎不是由滑膜细胞产生的。从慢性期的早期到中期，增殖的滑膜细胞产生IL-1β和IL-1ra。在关节炎的这一阶段，还观察到这些细胞因子在软骨细胞中形成簇。
结论
这个关节炎模型显示了关节炎症过程随时间的阶段性变化。IL-1β和IL-1ra是由某种细胞产生的，这取决于炎症的阶段。
BACKGROUND
In temporomandibular joint (TMJ) arthritis, there is limited knowledge of the relationship between interleukin-1beta (IL-1beta) and interleukin-1 receptor antagonist (IL-1ra), as well as the source of these cytokines. We investigated the development of an antigen-induced arthritis in the rabbit TMJ immunohistochemically.
METHODS
Unilateral TMJ arthritis was induced in 32 adult New Zealand White rabbits. From 6 h to 12 weeks after induction of arthritis, topology of IL-1beta and IL-1ra were observed.
RESULT
The acute stage of induced arthritis lasted for one week after induction, thereafter it became chronic. In the early phase of the acute stage, infiltrating inflammatory cells, as well as synovial cells, produced IL-1beta and IL-1ra. In the late phase of the acute stage, the main source of these cytokines was subsynovial fibroblasts. In this phase of arthritis, IL-1beta and IL-1ra did not appear to be produced by synovial cells. From the early to intermediate phase of the chronic stage, proliferating synovial cells produced IL-1beta and IL-1ra. In this phase of the arthritis, these cytokines were also observed in a cluster formation in chondrocytes.
CONCLUSION
This arthritis model shows a staging of the joint inflammation process with time. IL-1beta and IL-1ra are produced by a certain kind of cells depending on the stage of inflammation.
灭活淋病奈瑟菌和淋球菌脂多糖诱导家兔关节炎。
DOI：
--
发表时间：
1984
期刊：
The Journal of rheumatology
影响因子：
--
作者：
Goldenberg,DL;Reed,JI;Rice,PA
通讯作者：
Rice,PA