The need for speed in rodent locomotion analyses.
The need for speed in rodent locomotion analyses.
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DOI：
10.1002/ar.22955
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发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
Haulcomb MM
中科院分区：
文献类型：
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作者：
Batka RJ;Brown TJ;Mcmillan KP;Meadows RM;Jones KJ;Haulcomb MM
运动分析现在被广泛应用于许多动物物种中，以了解疾病中的运动缺陷，神经损伤后的功能恢复以及各种治疗的有效性。最近，啮齿动物运动分析已经成为一种越来越受欢迎的方法，在不同的研究范围。速度是运动的一个不可分割的方面，但仍然没有被完全理解，它的影响在分析数据时往往没有被适当地纳入。在这篇混合论文中，我们完成了三件事：(1)回顾了啮齿动物研究中速度和运动变量之间的相互作用；(2)使用CatWalk步态分析系统全面分析了16只野生型小鼠的速度和162个运动变量之间的关系；(3)开发和测试了一种在速度背景下分析和报告运动变量的统计方法。值得注意的结果包括：(1)CatWalk报告的90%以上的变量与速度相关，平均R2值为0.624;(2)大多数变量与速度的关系是非线性的；(3)目前控制速度的方法不足；(4)线性混合模型是一种适用于包含速度相关关系的运动分析的有效统计方法。鉴于运动变量对速度的普遍依赖，我们认为，除非在速度的背景下对运动分析进行分析和报告，否则无法得出有效的结论。
Locomotion analysis is now widely used across many animal species to understand the motor defects in disease, functional recovery following neural injury, and the effectiveness of various treatments. More recently, rodent locomotion analysis has become an increasingly popular method in a diverse range of research. Speed is an inseparable aspect of locomotion that is still not fully understood, and its effects are often not properly incorporated while analyzing data. In this hybrid manuscript, we accomplish three things: (1) review the interaction between speed and locomotion variables in rodent studies, (2) comprehensively analyze the relationship between speed and 162 locomotion variables in a group of 16 wild-type mice using the CatWalk gait analysis system, and (3) develop and test a statistical method in which locomotion variables are analyzed and reported in the context of speed. Notable results include the following: (1) over 90% of variables, reported by CatWalk, were dependent on speed with an average R2 value of 0.624, (2) most variables were related to speed in a nonlinear manner, (3) current methods of controlling for speed are insufficient, and (4) the linear mixed model is an appropriate and effective statistical method for locomotion analyses that is inclusive of speed-dependent relationships. Given the pervasive dependency of locomotion variables on speed, we maintain that valid conclusions from locomotion analyses cannot be made unless they are analyzed and reported within the context of speed.