Carriage of Cronobacter sakazakii in the Very Preterm Infant Gut
Carriage of Cronobacter sakazakii in the Very Preterm Infant Gut
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DOI：
10.1093/cid/ciy062
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发表时间：
2018-07-15
影响因子：
11.8
通讯作者：
Wylie, Todd N.
中科院分区：
文献类型：
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作者：
Chandrasekaran, Sukantha;Burnham, Carey-Ann D.;Wylie, Todd N.
背景坂崎克罗伊氏菌引起严重的新生儿感染，但我们对极低出生体重儿肠道携带这种病原体知之甚少。我们对来自圣刘易斯儿童医院的121名儿童的2304份粪便的16 S核糖体RNA（rRNA）基因进行了测序，这些儿童的出生体重为4.0%，在婴儿配方奶粉停止使用后，映射到克罗诺明的读数从4.3%下降到0.9%（P 99.9%序列同一性在他们所包含的大约93%的最佳匹配参考基因组中，并共享多个毒力位点。极低出生体重儿排出脓毒症致病性克罗伊氏菌。高密度的克隆测序样本在使用婴儿配方奶粉过程中更为常见。更好地了解婴儿肠道中克罗诺杆菌的生态将为未来的预防和控制策略提供信息。
Background. Cronobacter sakazakii causes severe neonatal infections, but we know little about gut carriage of this pathogen in very low birthweight infants.Methods. We sequenced 16S ribosomal RNA (rRNA) genes from 2304 stools from 121 children at St Louis Children's Hospital whose birthweight was 4.0% of reads mapping to Cronobacter fell from 4.3% to 0.9% after powdered infant formula was discontinued (P 99.9% sequence identity in the approximately 93% of best-match reference genome that they contained, and shared multiple virulence loci.Conclusions. Very low birthweight infants excrete putatively pathogenic Cronobacter. High-density Cronobacter sequence samples were more common during the use of powdered infant formula. Better understanding of the ecology of Cronobacter in infant guts will inform future prevention and control strategies.