TRANSPLANTATION OF LIVING NUCLEI FROM BLASTULA CELLS INTO ENUCLEATED FROGS EGGS
TRANSPLANTATION OF LIVING NUCLEI FROM BLASTULA CELLS INTO ENUCLEATED FROGS EGGS
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DOI：
10.1073/pnas.38.5.455
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发表时间：
1952-01-01
影响因子：
11.1
通讯作者：
KING, TJ
中科院分区：
文献类型：
--
作者：
BRIGGS, R;KING, TJ
本发明描述了一种用于将来自囊胚细胞的细胞核移植到先前去核的青蛙卵中的方法。蛙卵。刺但不去核的卵显示出败育型卵裂，而刺和去核的卵显示出无卵裂。638例眼球摘除术中99%成功。在498个从正常受精的去核卵中获得的胚胎中，除了一个外，其余都是单倍体，表明手术成功。当去核的尖音鸟卵注射二倍体尖音鸟囊胚细胞核超过一半分裂，并产生部分和完整的囊胚。74%的完整囊胚正常原肠胚，形成完整的胚胎，其中约一半是正常的，其余的略有异常。通过在不同阶段固定22个卵，获得了去除卵核的细胞学证据。由于已知萝卜与玉米杂交产生的胚为二倍体或单倍体。catesbeiana和R.为了验证移植技术的可行性，我们将牛肝菌细胞核移植到去核的尖音鸡卵中。供体囊胚是通过用catesbeiana精子使pipens卵受精并让部分卵发育为二倍体杂种而产生的，而在其他卵中，卵核被去除，产生雄核单倍体杂种。涉及二倍体和单倍体囊胚细胞的移植导致与对照组相同的死亡。杂种对照与实验的细胞学研究。囊胚显示出类似的异常，除了后者的3例，其中细胞核处于以弥漫性Feulgen染色为特征的异常间期状态。使用的核分化和功能的研究技术进行了讨论。
A method is described for transplanting a nucleus from a blastula cell into a previously enucleated frogs egg. Eggs of Rana pipiens. pricked but not enucleated, showed an abortive type of cleavage, whereas eggs pricked and enucleated showed no cleavage. 99% of 638 enucleations were successful. Of 498 embryos obtained from normally inseminated previously enucleated eggs, all but one were haploids, indicating the success of the operation. When enucleated pipiens eggs were injected with a diploid pipiens blastula cell nucleus more than half cleaved and gave rise to partial and complete blastulae. 74% of the complete blastulae gastrulated normally, forming complete embryos, of which about half were normal and the remainder slightly abnormal. Cytological evidence for removal of the egg nucleus was obtained by fixing 22 eggs at various stages. Since it was known that diploid or haploid embryos produced by crosses between R. catesbeiana and R. pipiens died at early stages of development, the transplantation technique was tested by placing catesbeiana nuclei in pipiens enucleated eggs. The donor blastulae were produced by inseminating pipens eggs with catesbeiana sperm and letting part of the eggs develop as diploid hybrids, whereas in others the egg nucleus was removed, giving androgenetic haploid hybrids. Transplantations involving both the diploid and haploid blastula cells resulted in death at the same time as the controls. Cytological studies of hybrid control and exptl. blastulae showed similar abnormalities, except for 3 cases in the latter where the nuclei were in an abnormal interphase condition characterized by diffuse Feulgen staining. Use of the technique for studies of nuclear differentiation and function is discussed.