Increased furanocoumarin production by Glehnia littoralis roots induced via Agrobacterium rhizogenes infection
Increased furanocoumarin production by Glehnia littoralis roots induced via Agrobacterium rhizogenes infection
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DOI：
10.5511/plantbiotechnology.11.0330a
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发表时间：
2011-01-01
影响因子：
1.6
通讯作者：
Kitamura, Yoshie
中科院分区：
文献类型：
--
作者：
Terato, Masahiro;Ishikawa, Aya;Kitamura, Yoshie
本研究的目的是确定一个可能的应用毛状根作为生物技术生产呋喃香豆素，通过建立根克隆北沙参感染发根农杆菌后。没有一个根克隆在无菌条件下生长，但当在吲哚-3-丁酸的存在下培养时，克隆间的生长变化很大。对10个克隆的致病性rol（A、B（TL）、B（TR）和C）基因的插入和表达分析表明，在2个克隆中检测到rolC的稳定表达和rolB的不规则表达，表现出活跃的生长和较高的呋喃香豆素诱导。抗坏血酸处理后，生产力的黄毒素和bergapten在根克隆窝藏致病基因的2.8和3.7倍以上，分别记录在野生型根。这些结果表明，具有致病性rol基因的根培养物提供了一种潜在的手段来生产呋喃香豆素。
The objective of this study was to determine a possible application of hairy roots as biotechnological producers of furanocoumarins, by establishing root clones of Glehnia littoralis after infection from Agrobacterium rhizogenes. None of the root clones grew under hormone-free conditions, but when cultured in the presence of indole-3-butyric acid, growth varied substantially across clones. Analysis of the insertion and expression of pathogenic rol (A, B(TL), B(TR), and C) genes for 10 clones showed that the stable expression of rolC and irregular expression of rolB were detected in 2 clones, which exhibited active growth and higher furanocoumarin induction. Following ascorbic acid treatment, the productivity of xanthotoxin and bergapten in a root clone harbouring a pathogenic gene was 2.8 and 3.7 times greater than that recorded in wild-type roots, respectively. These results indicate that root cultures possessing pathogenic rol genes offer a potential means to produce furanocoumarins.