Metabolic activity of Staphylococcus epidermidis is high during initial and low during late experimental foreign-body infection
Metabolic activity of Staphylococcus epidermidis is high during initial and low during late experimental foreign-body infection
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DOI：
10.1128/jb.186.8.2236-2239.2004
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发表时间：
2004-04-01
影响因子：
3.2
通讯作者：
Van Eldere, J
中科院分区：
文献类型：
--
作者：
Vandecasteele, SJ;Peetermans, WE;Van Eldere, J
众所周知，异物感染（FBI）对抗生素治疗的根除具有抗药性。据推测，降低的细菌代谢活性有助于这种抗性。我们研究了表皮葡萄球菌在体外异物定植过程中回收的204个样本和在大鼠模型中体内FBI过程中回收的424个样本中的代谢活性。通过定量PCR测定每个基因组16S rRNA的量来测量代谢活性。最初的异物相关生长被证明是一个代谢活跃的过程，在体外和体内。初始16S rRNA含量与体外指数生长期观察到的相似。然而，在体内FBI后期，观察到16S rRNA含量降低了114倍（P远小于0.0001），表明代谢活性显著降低。这种在晚期FBI期间代谢活动的减少至少可以部分解释为什么这种感染很难用常规抗生素治疗根除。
Foreign-body infection (FBI) is notoriously resistant to eradication by antibiotic treatment. It is hypothesized that reduced bacterial metabolic activity contributes to this resistance. We examined the metabolic activity of Staphylococcus epidermidis in 204 samples recovered during in vitro foreign-body colonization and in 424 samples recovered during in vivo FBI in a rat model. Metabolic activity was measured by determining the amount of 16S rRNA per genome by quantitative PCR. The initial foreign-body-associated growth proved to be a metabolically active process, both in vitro and in vivo. The initial 16S rRNA content was similar to that observed during in vitro exponential-growth phase. However, during late in vivo FBI, a 114-fold (P much less than 0.0001) decrease in the 16S rRNA content was observed, indicating that there was markedly decreased metabolic activity. This decreased metabolic activity during late FBI can explain at least in part why such infections are so difficult to eradicate with conventional antibiotic treatment.