Performance of a commercially available in-clinic ELISA for the detection of antibodies against Anaplasma phagocytophilum, Ehrlichia canis, and Borrelia burgdorferi and Dirofilaria immitis antigen in dogs
Performance of a commercially available in-clinic ELISA for the detection of antibodies against Anaplasma phagocytophilum, Ehrlichia canis, and Borrelia burgdorferi and Dirofilaria immitis antigen in dogs
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DOI：
10.2460/ajvr.71.12.1443
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发表时间：
2010-12-01
影响因子：
1
通讯作者：
Breitschwerdt, Edward B.
中科院分区：
文献类型：
--
作者：
Chandrashekar, Ramaswamy;Mainville, Celine A.;Breitschwerdt, Edward B.
目的-评估市售临床 ELISA 检测狗心丝虫感染和蜱传疾病的敏感性和特异性样本群体 - 从狗获得的 846 个血清血浆或血液样本程序 - 通过临床 ELISA 评估样本，以检测抗无形体吞噬埃里希体、伯氏疏螺旋体和恶丝虫（心丝虫）抗原的抗体。使用尸检结果对样品进行评估，对Dimmitis进行抗原测定，并针对针对伯氏疏螺旋体E cams和A phagocytophilum的抗体进行免疫荧光测定或蛋白质印迹分析结果-用于检测心丝虫抗原的临床ELISA的灵敏度和特异性（分别为99 2％和100％）针对伯氏疏螺旋体的抗体（分别为98 8％和100％）和针对E can's抗体（96分别为 2% 和 100%）与类似商业 ELISA 的结果相似 在从美国东北部和中西部北部的狗获得的样品中，与免疫荧光测定的结果相比，临床 ELISA 对无形体属抗体的敏感性和特异性分别为 99% 和 100% 通过使用临床 ELISA 测试了来自 2 只实验感染吞噬细胞菌 NY18 菌株的狗的样品接种后 8 天检测到抗 A 吞噬细胞球菌的抗体 实验感染狗体内的抗扁平无形体抗体与 A 吞噬细胞球菌分析物交叉反应 在一些犬血清样本中鉴定出双重感染 结论和临床相关性 - 兽医可使用临床上出售的 ELISA 来筛查狗是否有心丝虫感染以及是否暴露于蜱传病原体 (Am J Vet研究报告 2010 71 1443-1450)
Objective-To evaluate the sensitivity and specificity of a commercially available in-clinic ELISA for detection of heartworm infection and tick borne diseases in dogsSample Population-846 serum plasma or blood samples obtained from dogsProcedures-Samples were evaluated via the in-clinic ELISA to detect antibodies against Anaplasma phagocytophilum Ehrlichia can's and Borrelia burgdorferi and Dirofilaria immitis (heartworm) antigen True infection or immunologic status of samples was assessed by use of results of necropsy an antigen assay for D immitis and immunofluorescence assay or western blot analysis for antibodies against B burgdorferi E cams and A phagocytophilumResults-Sensitivity and specificity of the in-clinic ELISA for detection of heartworm antigen (99 2% and 100% respectively) antibodies against B burgdorferi (98 8% and 100% respectively) and antibodies against E can's (96 2% and 100% respectively) were similar to results for a similar commercial ELISA In samples obtained from dogs in the northeast and upper Midwest of the United States sensitivity and specificity of the in-clinic ELISA for antibodies against Anaplasma spp were 99 1% and 100% respectively compared with re suits for an immunofluorescence assay Samples from 2 dogs experimentally infected with the NY18 strain of A phagocytophilum were tested by use of the in clinic ELISA and anti bodies against A phagocytophilum were detected by 8 days after inoculation Antibodies against Anaplasma platys in experimentally infected dogs cross-reacted with the A phagocytophilum analyte Coinfections were identified in several of the canine serum samplesConclusions and Clinical Relevance-The commercially available in clinic ELISA could be used by veterinarians to screen dogs for heartworm infection and for exposure to tick-borne pathogens (Am J Vet Res 2010 71 1443-1450)