Ty virus-like particles, DNA vaccines and Modified Vaccinia Virus Ankara; Comparisons and combinations
Ty virus-like particles, DNA vaccines and Modified Vaccinia Virus Ankara; Comparisons and combinations
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DOI：
10.1515/bc.1999.041
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发表时间：
1999-03-01
影响因子：
3.7
通讯作者：
Hill, AVS
中科院分区：
文献类型：
--
作者：
Gilbert, SC;Schneider, J;Hill, AVS
三种疫苗都表达伯氏疟原虫的同一抗原，或该抗原的CD8(+)T细胞表位，比较了它们在小鼠体内诱导CD8(+)T细胞反应的能力。与DNA疫苗相比，TY病毒样颗粒和改良Vaccinia Virus Ankara(MVA)具有更高的裂解水平和更多的干扰素-γ分泌T细胞，但即使在重复接种后，这些疫苗也不能保护免疫小鼠免受感染攻击。然而，当免疫反应被启动时，一种类型的疫苗(Ty-VLP或DNA)和另一种(MVA)加强的疫苗实现了对感染的完全保护。这种疫苗接种方法使用可用于人类的递送系统和途径，并可为诱导高水平的CD8(+)T细胞提供普遍适用的机制。
Three types of vaccine, all expressing the same antigen from Plasmodium berghei, or a CD8(+) T cell epitope from that antigen, were compared for their ability to induce CD8(+) T cell responses in mice. Higher levels of lysis and numbers of IFN-gamma secreting T cells were primed with Ty virus-like particles and Modified Vaccinia Virus Ankara (MVA) than with DNA vaccines, but none of the vaccines were able to protect immunised mice from infectious challenge even after repeated doses. However, when the immune response was primed with,one type of vaccine (Ty-VLPs or DNA) and boosted with another (MVA) complete protection against infection was achieved. Protection correlated with very high levels of IFN-gamma secreting T cells and lysis, This method of vaccination uses delivery systems and routes that can be used in humans and could provide a generally applicable regime for the induction of high levels of CD8(+) T cells.