The application of NIR Raman spectroscopy in the assessment of serum thyroid-stimulating hormone in rats
The application of NIR Raman spectroscopy in the assessment of serum thyroid-stimulating hormone in rats
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DOI：
10.1016/j.saa.2004.03.016
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发表时间：
2005-01-01
影响因子：
4.4
通讯作者：
Sato-Berrú, R
中科院分区：
文献类型：
--
作者：
Medina-Gutiérrez, C;Quintanar, JL;Sato-Berrú, R
使用近红外拉曼光谱（830 nm）在铝基板上分析经促甲状腺激素释放激素（TRH）治疗的甲状腺功能正常和甲状腺切除的大鼠的血清样本。获得促甲状腺激素 (TSH)、TRH 和催乳素标准品的光谱。发现对照样品组和 Tx + TRH 样品组的拉曼光谱曲线之间存在差异。这些差异通过线性判别分析 (LDA) 得到证实，该分析在组之间提供了良好的分类。推测这些差异是由于甲状腺切除大鼠中 TSH 的增加而产生的。 (C) 2004 Elsevier B.V. 保留所有权利。
Serum blood samples of euthyroid and thyroidectomized rats treated with thyrotropin-releasing hormone (TRH) were analyzed on aluminum substrates using the near-infrared Raman spectroscopy (830 nm). Spectra of thyroid-stimulating hormone (TSH), TRH and prolactin standards were obtained. Differences between Raman spectra profiles of control and Tx + TRH samples groups were found. These differences were confirmed by the linear discriminant analysis (LDA), which presents a good classification between groups. It is supposed that these differences are produced by the increment of TSH in the thyroidectomized rats. (C) 2004 Elsevier B.V. All rights reserved.