Genetic variation during long-term preservation of bacteria in public culture collections.
Genetic variation during long-term preservation of bacteria in public culture collections.
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公共培养物保藏库中细菌长期保存过程中的遗传变异。
DOI：
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发表时间：
2018
影响因子：
2.8
通讯作者：
H. Kawasaki
中科院分区：
文献类型：
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作者：
Kenta Sakurai;H. Kawasaki
在长期保存过程中，已经观察到微生物菌株在培养集合（CCs）中的表型和遗传变化。必须验证这些变化对保存在CCs的菌株质量的影响。在这项研究中，我们对来自同一来源的不同生产批次进行了全基因组单核苷酸多态性（SNP）分析，这些批次在NITE生物资源中心（NBRC）通过4°C真空液体干燥方法保存了4-38年。对三组菌株进行分析，分别为纤维单胞菌NBRC 15513T、谷氨酸棒状杆菌NBRC 12168T和糖单孢菌病毒NBRC 12207T。为了进行比较，在所有被研究的集合中都发现了snp。在两个或三个批次的基因组序列中，在非编码序列（non-CDSs）和编码序列（CDSs）中都发现了基因组snp，并且CDSs中的snp导致了非同义突变。这些数据表明，基因组变异发生在长期保存过程中。
Phenotypic and genetic changes during long-term preservation have been observed in microbial strains at culture collections (CCs). It is imperative to verify the effects of these changes on quality of the strains preserved at CCs. In this study, we performed genome-wide single-nucleotide polymorphism (SNP) analysis of different production lots, which had been derived from the same origin and preserved at the NITE Biological Resource Center (NBRC) for a 4-38-year period by the vacuum liquid drying method at 4 °C. The analysis was conducted for three sets of lots derived from Cellulomonas fimi NBRC 15513T, Corynebacterium glutamicum NBRC 12168T, and Saccharomonospora viridis NBRC 12207T. SNPs were found in all sets studied for comparison purposes. In sets of two or three lots, genomic SNPs were found in both non-coding sequences (non-CDSs) and in coding sequences (CDSs), and the SNPs in the CDSs resulted in non-synonymous mutations. These data indicated that genomic variation occurred during long-term preservation.