Somatic embryogenesis and shoot regeneration induced in cucumber leaves
Somatic embryogenesis and shoot regeneration induced in cucumber leaves
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发表时间：
2010
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通讯作者：
M. Usman;Z. Hussain;And B. Fatima
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作者：
M. Usman;Z. Hussain;And B. Fatima
以黄瓜品种为材料，研究了植物生长调节剂（PGRs）对黄瓜胚状体发生和植株再生的影响。叶盘外植体在MS +2，4-D（2 mgL-1）+NAA（1.5mgL-1）+BAP（1.5mgL-1）的培养基上愈伤组织诱导率最高，分别为94.16%和76%。种子子叶外植体在4.0 + 0.75mgL-1（BAP + NAA）的培养基上愈伤组织诱导率最高（77%）。在最高浓度的2，4-D（5 mgL-1）诱导的叶盘愈伤组织中，胚形成率最高（23%），而在BAP和BAP + NAA（5 + 1 mgL-1）诱导的愈伤组织中，再生芽的比例分别为14%和12%。切下这些芽并在MSO培养基上生根。试管苗移栽到盆中，驯化后移栽到田间。所开发的植物材料将在形态学和遗传学上表征纯合性。
Commercial cucumber cultivars were explored for embryogenesis and plant regeneration induced in somatic tissues on plant growth regulators (PGRs). Maximum callus induction 94.16% and 76% was observed in leaf disc explants on MS medium supplemented with 2,4-D (2 mgL -1 ), NAA and BAP (1.5 mgL -1 , each), respectively. Seed cotyledon explants induced maximum calli (77%) on 4.0 + 0.75 mgL -1 (BAP + NAA, respectively). Calli induced in leaf disc on the highest level of 2,4-D (5 mgL -1 ) yielded the highest embryo formation (23%) whereas calli induced on BAP and BAP + NAA (5 + 1 mgL -1 ) regenerated into 14% and 12%, shoots respectively. These shoots were excised and rooted on MSO medium. The plantlets were transplanted in pots and transferred to field after acclimatization. The developed plant material will be morphologically and genetically characterized for homozygosity.