Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP+-dependent xylitol dehydrogenase, and xylulokinase
Bioethanol production from xylose by recombinant Saccharomyces cerevisiae expressing xylose reductase, NADP+-dependent xylitol dehydrogenase, and xylulokinase
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DOI：
10.1263/jbb.105.296
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发表时间：
2008-03-01
影响因子：
2.8
通讯作者：
Sawayama, Shigeki
中科院分区：
文献类型：
--
作者：
Matsushika, Akinori;Watanabe, Seiya;Sawayama, Shigeki
构建了一批具有木糖发酵能力的重组酿酒酵母菌株。重组S.与表达野生型XDH的参考菌株D-XR/XDH/XK相比，其中表达蛋白质工程化NADP（+）依赖性XDH的酿酒酵母菌株D-XR/ARSdR/XK显示出40%的乙醇产量增加和23%的木糖醇分泌减少。
We constructed a set of recombinant Saccharomyces cerevisiae strains with xylose-fermenting ability. A recombinant S. cerevisiae strain D-XR/ARSdR/XK, in which protein engineered NADP(+)-dependent XDH was expressed, showed 40% increased ethanol production and 23% decrease in xylitol excretion as compared with the reference strain D-XR/XDH/XK expressing the wild-type XDH.