DNA Supercoiling and Repair in Peripheral Lymphocytes as a Measure of Acute Radiation Response After Radiotherapy.
DNA Supercoiling and Repair in Peripheral Lymphocytes as a Measure of Acute Radiation Response After Radiotherapy.
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外周淋巴细胞中 DNA 超螺旋和修复作为放射治疗后急性放射反应的衡量标准。
DOI：
10.1002/roi.2970020304
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Abel,Helmut
中科院分区：
文献类型：
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作者：
Rosemann,Michael;Schulze,Brigitte;Abel,Helmut
对20例肿瘤患者和17例健康供者的淋巴细胞进行了紫外线（UV）切除修复过程中DNA超螺旋密度和切口动力学的测量。使用了类核沉降，它可以灵敏地检测DNA损伤和染色质结构的改变。比较了肿瘤患者和正常供体淋巴细胞经溴化乙啶插入后DNA超螺旋的释放和紫外线照射后切口步骤的动力学。将淋巴细胞分别分为修复正常或减少和超旋回正常或改变，结果显示，来自患者的淋巴细胞（分别为40%和85%）比来自健康供体的淋巴细胞（分别为35%和23%）更频繁地发生修复减少和染色质结构改变。更惊人的是，这两种特征在肿瘤患者中同时出现：在所有病例中，34%的修复减少与超螺旋密度改变有关，而在健康供体中，这种关联仅在所有病例中占18%。超卷曲密度可能与淋巴细胞的功能完整性和辐射损伤后的修复能力有关。由于这两个参数对正常组织的辐射反应都很重要，我们认为这些测量是一种潜在的预后测定，旨在减少正常组织的急性反应。©Wiley‐Liss, Inc。
DNA supercoiling density and incision kinetics during ultraviolet (UV) excision repair hav been measured in lymphocytes from 20 cancer patients and 17 healthy donors. Nucleoid sedimentation was used, which allows the sensitive detection of both DNA damage and alterations in chromatin structure. The release of DNA supercoiling after ethidium bromide intercalation and the kinetics of the incision step following UV irradiation were compared in lymphocytes derived from cancer patients and those from normal donors. The classification into lymphocytes with normal or reduced repair and normal or altered supercoiling, respectively, revealed that reduced repair as well as altered chromatin structure occurred more frequently in lymphocytes derived from patients (40% and 85%, respectively) than in those from healthy donors (35% and 23%, respectively). Even more striking was the simultaneous occurrence of both characteristics in tumor patients: in 34% of all cases reduced repair was associated with altered supercoiling density, whereas among healthy donors this association occurred in only 18% of all cases. Supercoiling density may be related to functional integrity of lymphocytes and repair capacity to recovery after radiation damage. Since both parameters are important for the radiation response of normal tissue, we consider these measurements a potential prognostic assay aimed at reducing acute reaction of the normal tissue. © Wiley‐Liss, Inc.