Matrix effects in analysis of dialkyl phosphate metabolites of organophosphate pesticides in urine by gas chromatography/tandem mass spectrometer.
Matrix effects in analysis of dialkyl phosphate metabolites of organophosphate pesticides in urine by gas chromatography/tandem mass spectrometer.
复制标题
气相色谱/串联质谱仪分析尿液中有机磷农药的磷酸二烷基酯代谢物时的基质效应。
DOI：
10.1080/03601234.2013.730021
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发表时间：
2013
期刊：
影响因子：
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通讯作者：
She,Jianwen
中科院分区：
文献类型：
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作者：
Wang,Dongli;Behniwal,Paramjit;Fan,Ruifang;SimonIp,HoSai;She,Jianwen
尿二烷基磷酸酯代谢物（DAP）被用作生物标志物，以评估人体暴露于有机磷农药。目的是评价样品制备和方法校准期间尿液DAP分析中的潜在伪影。稀释的尿液池通常用于制备校准标准品，以尽量减少由于尿液基质复杂性造成的影响。通过将已知量的标准品加标至蒸馏水、合成尿液和稀释尿液池中，评价了对DAP测量的基质效应。不同基质导致所有目标化合物的检测浓度相似，但磷酸二甲酯（DMSO）除外，其测量偏差高达加标量的8倍。在本研究中，我们还发现，尿液颗粒，通常出现在解冻后的冷冻人尿液样本，可以影响DAP的测量，特别是双磷酸和二乙基硫代磷酸（DETP）。比较了三种类型的样本基质（即无颗粒尿液、含颗粒尿液和仅含颗粒尿液）中的DAP测量结果。DETP在此准备步骤中可能会出现较大误差。还评价了使用氘代和13C12标记的DAP作为内标。总的来说，这些问题可能会导致错误识别和不准确，这可能会严重影响数据质量。
Urinary dialkyl phosphate metabolites (DAPs) are used as biomarkers to evaluate human exposure to organophosphate pesticides. The objective was to evaluate potential artifacts in urinary DAPs analysis during sample preparation and method calibration. Diluted urine pools were commonly used to prepare calibration standards to minimize the effects due to the complexity of urine matrix. Matrix effects on measurements of DAPs were evaluated by spiking known amount of standards into distilled water, synthetic urine and diluted urine pool. Different matrices resulted in similar concentrations detected for all target compounds, except dimethylphosphate (DMP) with the deviation of measurement as large as eight times the spiked amount. In this study, we also found that urinary particles, which usually appeared after thawing frozen human urine samples, could affect the measurements of DAPs, especially DMP and diethylthiophosphate (DETP). Results of DAPs measurements in three types of sample matrices, i.e. urine without particles, urine with particles and particles only were compared. DETP could be subject to large error during this preparation step. The use of deuterated and13C12-labeled DAPs as internal standards is also evaluated. Overall, these issues can cause misidentification and inaccuracies, which may significantly affect the data quality.