Bactericidal Effect of Strong Acid Electrolyzed Water against Flow Enterococcus faecalis Biofilms
Bactericidal Effect of Strong Acid Electrolyzed Water against Flow Enterococcus faecalis Biofilms
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强酸电解水对流动粪肠球菌生物膜的杀菌效果
DOI：
10.1016/j.joen.2016.04.009
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发表时间：
2016-07-01
影响因子：
4.2
通讯作者：
Yu, Qing
中科院分区：
文献类型：
--
作者：
Cheng, Xiaogang;Tian, Yu;Yu, Qing
摘要：本研究评价了强酸电解水（SAEW）对流动粪肠球菌生物膜的杀菌效果及其作为根管冲洗剂的潜在应用前景。方法：在微流控系统中恒剪切流制备粪肠杆菌生物膜。为了比较，在盖层表面静态条件下生成静态粪肠球菌生物膜。流动和静态粪肠球菌生物膜均采用SAEW处理。次氯酸钠（NaOCl, 5.25%）和生理盐水（0.9%）作为对照。使用共聚焦激光扫描显微镜和细胞计数法评估细菌减少量。用扫描电镜观察细菌细胞的形态变化。结果：激光共聚焦扫描显微镜和细胞计数结果表明，SAEW对流动和静态粪肠球菌生物膜的杀菌效果与5.25% NaOCl相似。扫描电镜结果显示，经SAEW处理后，光滑、连续、明亮的细菌表面变得粗糙、萎缩，甚至裂解，与NaOCl组相似。结论：SAEW对流动型和静态型粪肠杆菌生物膜均有较好的杀菌效果，可作为根管冲洗剂进行有效的根管消毒。
Introduction: This study evaluated the bactericidal effect of strong acid electrolyzed water (SAEW) against flow Enterococcus faecalis biofilm and its potential application as a root canal irrigant. Methods: Flow E. faecalis biofilms were generated under a constant shear flow in a microfluidic system. For comparison, static E. faecalis biofilms were generated under a static condition on coverslip surfaces. Both the flow and static E. faecalis biofilms were treated with SAEW. Sodium hypochlorite (NaOCl, 5.25%) and normal saline (0.9%) were included as the controls. Bacterial reductions were evaluated using confocal laser scanning microscopy and the cell count method. Morphological changes of bacterial cells were observed using scanning electron microscopy. Results: The confocal laser scanning microscopic and cell count results showed that SAEW had a bactericidal effect similar to that of 5.25% NaOCl against both the flow and static E. faecalis biofilms. The scanning electron microscopic results showed that smooth, consecutive, and bright bacteria surfaces became rough, shrunken, and even lysed after treated with SAEW, similar to those in the NaOCl group. Conclusions: SAEW had an effective bactericidal effect against both the flow and static E. faecalis biofilms, and it might be qualified as a root canal irrigant for effective root canal disinfection.