FudC, a protein primary responsible for furfural detoxification in Corynebacterium glutamicum
FudC, a protein primary responsible for furfural detoxification in Corynebacterium glutamicum
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FudC，一种主要负责谷氨酸棒杆菌中糠醛解毒的蛋白质
DOI：
10.1007/s00253-015-7115-y
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发表时间：
2016
影响因子：
5
通讯作者：
Kondo A
中科院分区：
文献类型：
--
作者：
Tsuge Y;Kudou M;Kawaguchi H;Ishii J;Hasunuma T;Kondo A
木质纤维素水解物含有抑制微生物生长和发酵的化合物，从而降低生物燃料和生化生产的生产率。特别是，杂环醛糠醛是在这些水解物中发现的毒性最大的化合物之一。我们之前已经证明谷氨酸棒状杆菌可以将糠醛转化为毒性较小的化合物糠醇和2-糠酸。然而，迄今为止，参与这些氧化和还原反应的基因尚未在c中确定。glutamicumgenome。在这里，我们发现Cgl0331（指定为FudC）主要负责将糠醛还原成糠醇。编码FudC基因的缺失显著减少了体内糠醛还原为糠醇的过程。从大肠杆菌中纯化的his标记的FudC蛋白也被证明在体外反应中利用NADPH（而不是NADH）作为辅助因子将糠醛转化为糠醇。动力学测量表明，与负责糠醛还原的蛋白inE相比，FudC对糠醛具有高亲和力，但对其他醛的底物范围较窄。杆菌。
Lignocellulosic hydrolysates contain compounds that inhibit microbial growth and fermentation, thereby decreasing the productivity of biofuel and biochemical production. In particular, the heterocyclic aldehyde furfural is one of the most toxic compounds found in these hydrolysates. We previously demonstrated thatCorynebacterium glutamicumconverts furfural into the less toxic compounds furfuryl alcohol and 2-furoic acid. To date, however, the genes involved in these oxidation and reduction reactions have not been identified in theC. glutamicumgenome. Here, we show that Cgl0331 (designated FudC) is mainly responsible for the reduction of furfural into furfuryl alcohol inC. glutamicum. Deletion of the gene encoding FudC markedly diminished the in vivo reduction of furfural to furfuryl alcohol. Purified His-tagged FudC protein fromEscherichia coliwas also shown to convert furfural into furfuryl alcohol in an in vitro reaction utilizing NADPH, but not NADH, as a cofactor. Kinetic measurements demonstrated that FudC has a high affinity for furfural but has a narrow substrate range for other aldehydes compared to the protein responsible for furfural reduction inE. coli.